Preparation and Characterization of Polycaprolactone / Layered Double Hydroxide Nanocomposite for Hard Tissue Engineering Applications

Authors

1 1- Stem cell and Regenerative Medicine Department, Institute of Medical biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

2 2- Biomaterial Group, Biomedical Engineering Faculty, Amirkabir University of Technology, Tehran, Iran

3 3- Physics Group, Faculty of Science, Imam Khomeini International University, Qazvin, Iran

Abstract

In recent years the use of nanomaterials in bone tissue engineering scaffold has been considered due to its imitating the structure of natural bone tissue which contains a nanocomposite structure mixed with a three-dimensional matrix. In the meantime, Polycaprol actone has been used as a bio-polymer in bone tissue engineering applications as a scaffold. The aim of this study is to develop porous scaffolds made of polycaprol actone/layered double hydroxide biocomposite, with appropriate mechanical, bioactive and biological properties, for bone tissue engineering application. The nanocomposite scaffolds were fabricated by the particulate leaching method and freeze-drying method. In this study, MG63 cells (osteosarcoma) was investigated for cellular study. Energy dispersive X-ray analysis confirmed uniform distribution of ceramic phase in polycaprol actone matrix. The results of mechanical tests showed the increase in young’s modulus after addition of ceramic phase. The microscopic investigations demonstrated that the pores generated after addition of ceramic phase and the average size of pores was as large as 100-600μm. Also by the addition of LDH, the hydrophilicity of PCL increased but the rate of hydroxyapatite formation was delayed due to presence of magnesium ions. The cell culture experiments confirmed the attachment and proliferation of cells on the scaffolds. The results showed that the fabricated scaffolds have the potential to be used in cancellous bone tissue engineering.

Keywords

References

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Journal of Advanced Materials in Engineering (Esteghlal)
Volume 35, Issue 3
December 2016
Pages 13-28

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