Poly (hydroxybutyrate co hydroxyvalerate) Nanofibrous Scaffold Containing HydroxyapatiteBredigite Nanoparticles: Characterization and Biological Evaluation

Authors

1 1. Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.

2 2. Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, National University of Singapore, Singapore.

Abstract

In this work, poly (hydroxybutyrate co hydroxyvalerate) (PHBV) composite nanofibrous scaffold containing hydroxyapatite/bredigite (HABR) nanoparticles was fabricated through electrospining method. The morphology of prepared  nanofibers and the state of the nanoparticles dispersion in nanofiber matrix were investigated using scanning and transmission electron microscopy, respectively. Evaluation of the mechanical properties of the nanofibrous scaffolds revealed that there is a limit to the nanoparticle concentration at which nanoparticles can improve the mechanical properties of the nanofibrous scaffolds. According to the results, PHBV/HABR nanofibers showed higher wettability compared to PHBV nanofibers. In vitro cell culture assay was done using human fetal osteoblast cells on nanofibrous scaffold. MTS assay revealed that cell proliferation on the composite nanofibrous scaffold was significantly higher than those on the pure scaffold after 10 and 15 days. Scanning electron microscopy- Energy dispersive X-ray spectroscopy and CMFDA colorimeter assay analysis showed that the cells on the PHBV/HABR scaffolds acquired higher mineral deposition than the cells on the pure PHBV and control sample scaffold. Based on the results we concluded that PHBV/HABR nanofibers scaffold with higher wettability, improved mechanical properties and cell behavior hold great potential in bone regeneration applications.

Keywords

References

1. Puppi, D., Chiellini, F., Piras, A. M., and Chiellini. E., “Polymeric Materials for Bone and Cartilage Repair”, Progress in Polymer Science, Vol. 35, pp. 403-440, 2010.
2. Zhang, Z., Hu, J., and Ma, P. X., “Nanofiber-Based Delivery of Bioactive Agents and Stem Cells to Bone Sites”, Advanced Drug Delivery Review, Vol. 65, pp. 1129-1141, 2012.
3. Abdal-hay, A., Tijing, L. D., and Lim, J. K., “Characterization of the Surface Biocompatibility of an Electrospun Nylon 6/CaP Nanofiber Scaffold using Osteoblasts”, Chemical Engineering Journal, Vol. 215-216, pp. 57-64, 2013.
4. Holzwarth, J. M., and Ma, P. X., “Biomimetic Nanofibrous Scaffolds for Bone Tissue Engineering”, Biomaterials, Vol. 32, No. 36, pp. 9622-9629, 2011.
5. Tian, H., Tang, Z., Zhuang, X., Chen, X., and Jing, X., “Biodegradable Synthetic Polymers: Preparation, Functionalization and Biomedical Application”, Progress in Polymer Science, Vol. 37, No. 2, pp. 237-280, 2012.
6. Rezwan, K., Chen, Q. Z., Blaker, J. J., and Boccaccini, A. R., “Biodegradable and Bioactive Porous Polymer/Inorganic Composite Scaffolds for Bone Tissue Engineering”, Biomaterials, Vol. 24, pp. 3413-3431, 2006.
7. Chena, G. Q., and Wu, Q., “The Application of Polyhydroxyalkanoates as Tissue Engineering Materials”, Biomaterials, Vol. 26, pp. 6565-6657, 2005.
8. Kouhi, M., Morshed, M., Varshosaz, J., and Fathi, M. H., “Poly (e-caprolactone) Incorporated Bioactive Glass Nanoparticles and Simvastatin Nanocomposite Nanofibers: Preparation, Characterization and In Vitro Drug Release for Bone Regeneration Applications”, Chemical Engineering Journal, Vol. 228, pp. 1057-1065, 2013.
9. Webster, T. J, Siegel, R. W., and Bizios, R., “Osteoblast Adhesion on Nanophase Ceramics”, Biomaterials, Vol. 20, No. 13, pp. 1221-1227, 1999.
10. Hench, L. L., and Polak, J. M. “Third-Generation Biomedical Materials”, Science, Vol. 295, pp. 1014-1017, 2012.
11. Hench, L. L, “Bioceramics: from Concept to Clinic”, Journal of American Ceramic Society, Vol. 74, pp. 1485-1510, 1991.
12. Suchanek, W., and Yoshimura, M., “Processing and Properties of Hydroxyapatite-Based Biomaterials for Use as Hard Tissue Replacement Implants”, Journal of Materials Research, Vol. 13, pp. 94-117, 1998.
13. Taherian, M., Rojaee, R., Fathi, M., and Tamizifar, M., “Effect of Different Sol-Gel Synthesis Processes on Microstructural and Morphological Characteristics of Hydroxyapatite-Bioactive Glass Composite Nanopowders”, Journal of Advanced Ceramic, Vol. 3, No. 3, pp. 207-214, 2014.
14. Lin, K., Chang, J., Liu, X., and Ning, C. “Synthesis and Characterization of Nanocomposite Powders Composed of Hydroxyapatite Nanoparticles and Wollastonite Nanowires”, International Journal of Applied Ceramic Technology, Vol. 7, pp. 178-183, 2010.
15. Ohtsuki, C., Kokubo, T., and Yamamuro, T., “Mechanism of Apatite Formation on CaO-SiO2-P2O5 Glasses in a Simulated Body Fluid”, Journal of Non-Crystal Solids, Vol. 143, pp. 84-92, 1992.
16. Valerio, P., Pereira, M. M., Goes, A. M., and Leite, M. F., “The Effect of Ionic Products from Bioactive Glass Dissolution on Osteoblast Proliferation and Collagen Production”, Biomaterials, Vol. 25, pp. 2941-2948, 2004.
17. Wu, C., Chang, J., Zhai, W., and Ni, S., “A Novel Bioactive Porous Bredigite (Ca7MgSi4O16) Scaffold with Biomimetic Apatite Layer for Bone Tissue Engineering”, Journal of Materials Science: Materials in Medicine, Vol. 18, No. 5, pp. 857-864, 2007.
18. Wu, C., and Chang, J. “Degradation, Bioactivity, and Cytocompatibility of Diopside, Akermanite, and Bredigite Ceramics”, Journal of Biomedical Materials Research B, Vol. 83B, No.1, pp. 153-160, 2007.
19. Kouhi, M., Shamanian, M., Fathi, M., Samadikuchaksaraei, A., and Mehdipour, A., “Synthesis, Characterization, In Vitro Bioactivity and Biocompatibility Evaluation of Hydroxyapatite/Bredigite (Ca7 MgSi4 O16 ) Composite Nanoparticles”, JOM, Vol. 68, 1061-1070, 2016
20. Zhang, S., Prabhakaran, M. P., Qin, X., and Ramakrishna, S., “Poly 3-hydroxybutyrate-co-3-hydroxyvalerate Containing Scaffolds and Their Integration with Osteoblast as a Model for Bone Tissue Engineering”, Journal of Biomaterials Applications, Vol. 10, pp. 1394-1406, 2015.
21. Zhang, S., Prabhakaran, M. P., Qin, X., and Ramakrishna, S., “Biocomposite Scaffold for Bone Regeneration: Role of Chitosan and Hydroxyapatite within Poly 3-hydroxybutyrate-co-3-hydroxyvalerate on Mechanical Properties and In Vitro Evaluation”, Journal of the Mechanical Behavior of Biomedical Materials, Vol. 51, pp. 88-98, 2015.
22. Pascu, E., Stokes, J., and McGuinness, G. B., “Electrospun Composites of PHBV, Silk Fibroin and Nano-Hydroxyapatite for Bone Tissue Engineering”, Materials Science and Engineering C, Vol. 33, pp. 4905-4916, 2013.
23. LU, L. X., Wang, Y. Y., Mao, X., Xiao, Z. D., and Huang, N. P., “The Effects of PHBV Electrospun Fibers with Different Diameters and Orientations on Growth Behavior of Bone Marrowderived Mesenchymal Stem Cells”, Journal of Biomedical Materials, Vol. 7, pp. 1-10, 2012.
24. Zhang, N., Xiao, Q. R., Man, X. Y., Liu, H. X., LU, L. X., and Huang, N. P., “Spontaneous Osteogenic Differentiation of Mesenchymal Stem Cells on Electrospun Nanofibrous Scaffolds”, ARC Advances, Vol. 6, pp. 22144-22152, 2016.
25. Sajesh, K. M., Kiran, K., Nair, S. V., and Jayakumar, R., “Sequential Layer-by-Layer Electrospinning of Nano SrCO /PRP loaded PHBV Fibrous Scaffold For Bone Tissue Engineering”, Composites Part B, Vol. 99, pp. 445-452, 2016.
26. Wutticharoenmongkol, P., Sanchavanakit, N., Pavasant, P., and Supaphol, P., “Preparation and Characterization of Novel Bone Scaffolds Based on Electrospun Polycaprolactone Fibers Filled with Nanoparticles”, Macromolecular Bioscience, Vol. 6, pp. 70-77, 2006.
27. Erisken, C., Kalyon, D. M., and Wang, H., “Functionally Graded Electrospun Polycaprolactone and B-Tricalcium Phosphate Nanocomposites for Tissue Engineering Applications”, Biomaterials, Vol. 29, pp. 4065-4073, 2008.
28. Sultana, N., and Wang, M., “PHBV/PLLA-Based Composite Scaffolds Fabricated using an Emulsion Freezing/Freeze-Drying Technique for Bone Tissue Engineering: Surface Modification and In Vitro Biological Evaluation”, Biofabrication, Vol. 4, pp. 1317-1324, 2012.
29. Lee, E. J., Teng, S. H., Jang, T. S., Wang, P., Yook, S. W., and Kim, H. E., “Nanostructured Poly(e-caprolactone)-Silica Xerogel Fibrous Membrane for Guided Bone Regeneration”, Acta Biomaterialia, Vol. 6, pp. 3557-3565, 2010.
30. Han, L., Han, C., Cao, W., Wang, X., Bian, J., and Dong, L., “Preparation and Characterization of Biodegradable Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/ Silica Nanocomposites”, Polymer Engineering Science, Vol. 52, No. 2, pp. 250-258, 2012.
31. Causa, F., Netti, P. A., Ambrosio, L., Ciapetti, G., Baldini, N., Pagani, S., Martini, D., and Giunti, A., “Poly-e-caprolactone/Hydroxyapatite Composites for Bone Regeneration: In Vitro Characterization and Human Osteoblast Response”, Journal of Biomedical Materials Research, Vol. 76A, pp. 151-162, 2006.
32. Jose, M. V.,Thomas, V., Johnson, K. T., Dean, D. R., and Nyairo, E., “Aligned PLGA/HA Nanofibrous Nanocomposite Scaffolds for Bone Tissue Engineering”, Acta Biomaterialia, Vol. 5, pp 305-315, 2009
33. Ramier, J., Bouderlique, T., Ne, O., Renard, E., Albanese, P., and Grande, D., “Biocomposite Scaffolds Based on Electrospun Poly(3-hydroxybutyrate) Nanofibers and Electrosprayed Hydroxyapatite Nanoparticles for Bone Tissue Engineering Applications”, Materials Science and Engineering, Vol. 1, No. 38, pp. 161-169, 2014.
34. Porter, A. E., Buckland, T., Hing, K., Best, S. M, and Bonfield, W., “The Structure of the Bond Between Bone and Porous Silicon-Substituted Hydroxyapatite Bioceramic Implants”, Journal of Biomedical Materials Research, Vol. 78A, pp. 25-33, 2006.
35. Zhang, Y., Venugopal, J. R., El-Turki, A., Ramakrishna, S., Su, B., and Lim, C. T., “Electrospun Biomimetic Nanocomposite Nanofibers of Hydroxyapatite/Chitosan for Bone Tissue Engineering”, Biomaterials, Vol. 29, No. 32, pp. 4314-4322, 2008.
36. Prabhakaran, M. P., Venugopal, J., and Ramakrishna, S., “Electrospun Nanostructured Scaffolds for Bone Tissue Engineering”, Acta Biomaterialia, Vol. 5, No. 8, pp. 2884-2893, 2009.
37. Wu, C., Wang, J., Ni, S., and Zhai, W., “Preparation and Characteristics of a Calcuim Magnesium Silicate (Bredigite) Bioactive Ceramic”, Biomaterials, Vol. 26, No. 16, pp. 2925-2931, 2006.
38. Soulie, J., Nedelec, J. M., and Jallot, E., “Influence of Mg Doping on the Early Steps of Physico-Chemical Reactivity of Sol-Gel derived Bioactive Glasses in Biological Medium”, Physical Chemistry Chemical Physics, Vol. 11, No. 44, pp. 1043-1048, 2009.
Journal of Advanced Materials in Engineering (Esteghlal)
Volume 36, Issue 3
November 2017
Pages 87-99

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