Fabrication and Characterization of Polycaprolactone – Zeolite Y Nanocomposite for Bone Tissue Engineering

Authors

1 Department of Materials and Metallurgical Engineering, Iran University of Science and Technology, Narmak, Tehran 16846-13114, Iran.

2 Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.

Abstract

In recent years, nanoceramics have been used in scaffolds to emulate the nanocomposite with a three-dimensional structure of natural bone tissue. In this regard, polycaprolactone biopolymer is widely used as a scaffold in bone tissue engineering. The goal of this research is to produce porous scaffolds of polycaprolactone - zeolite biocomposite with suitable mechanical, bioactive and biological properties  for bone tissue engineering applications. The nanocomposite scaffolds were synthesized by solvent casting/particulate leaching and freeze-drying approaches. Microscopic investigations showed generation of pores with an average size of 200-400μm after addition of ceramic phase. Energy dispersive X-ray analysis confirmed uniform distribution of ceramic phase in polycaprolactone matrix. FTIR results determined the binding type of zeolite nanoparticles to the polycaprolactone matrix as physical bonding. The results of mechanical tests showed the increase in young’s modulus after addition of ceramic phase (from 0.04 to 0.3 and 3 to 7 MPa, respectively). The hydrophilicity of polycaprolactone increased after adding nanozeolite and more weight loss was observed for scaffold containing 20% zeolite (53.52 6 1.6%) with an increase in the rate of hydroxyapatite formation. The results showed that the prepared scaffolds have potential for cancellous bone tissue engineering application.

Keywords

References

1. Hing, K., “Bone Repaire in the Twenty- First Century: Biology, Chemistry or Engineering”, Philosophcal Transaction: Mathematical, Physical and Engineering Science, Vol. 62, pp. 2821-2850. 2004.
2. Bronner, F., Farach-C, M. C., and Mikos, A., Engineering of Functional Skeletal Tissues, Springer, Londen, 2007.
3. Ma, P. X., “Scaffolds for Tissue Fabrication”, Materialstoday, pp. 30-40, 2004.
4. Stamatialis, F. D., Papenburg, J. B., Girones, M., Saiful, S., Bettahalli, N. M. S., Schmitmeier, S., and Wessling, M., “Medical Application of Membrances: Drug Delivery, Artificial Organs and Tissue Engineering”, Journal of Membrane Science, Vol. 308, pp. 1-34, 2008.
5. Fergal, J., and Brien, O., “Biomaterials & Scaffolds for Tissue Engineering”, Materialstoday, Vol. 14, No. 3, pp. 88-95, 2011.
6. Lukasz, J., Mateusz, J., and Slawomir, S., “Designing of Macroporous Magnetic Bioscaffold Based on Functionalized Methacrylate Network Covered by Hydroxyapatites and Doped with Nano-MgFe2O4 for Potential Cancer Hyperthermia Therapy”, Materials Science & Engineering, Vol. 78, No. 1, pp. 901-911, 2017.
7. Ning, Ch., Cheng, H., Yin, Zh., Zhu, W., Chen, H., Lei, Sh., Yin, Sh., and Liu, H., “Preparation of Porous Polycaprolactone Scaffolds by using Freeze-Drying Combined Porogen-leaching Methods”, Key Engineering Materials, Vols. 368-372, pp. 1224-1226, 2008.
8. Sultanova, Z., Kaleli, G., Kabay, G., and Mutlu, M., “Controlled Release of a Hydrophobic Drug Coaxially Electrospun Polycaprolacton”, International Journal of Pharmaceutics, Vol. 505, No. 1-2, pp. 133-138, 2016.
9. Woodruff, M., and Hutmacher, D., “The Return of a Forgotten Polymer -Polycaprolactone in the 21st Century”, Progress in Polymer Science, Vol. 35, 10, pp. 1217-1256, 2010.
10. Zhou, Z., Zhou, Y., Chen, Y., Nie, H., Wang, Y., Li, F., and Zheng, Y., “Bilayer Porous Scaffold Based on Poly-(-caprolactone) Nanofibrous Membrane and Gelatin Sponge for Favoring Cell Proliferation”, Applied Surface Science, Vol. 258, pp. 1670 -1676, 2011.
11. Khatamian, M., Yavari, A., Akbarzadeh, A., and Saket Oskoui, M., “A Study on the Synthesis of [Fe, B]-MFI Zeolites using Hydrothermal Method and Investigation of their Properties”, Journal of Molecular Liquids, Vol. 242, pp. 979-986, 2017.
12. Joseph, F., “Synthesis of Linde -Type X Zeolite and its Application to Imprrove Soil Nutrients”, Master of Scince, Departemant of Physics, Kwame Nkrumah University of Scince and Tecnology, 2011.
13. Auerbach, S., Carrado, K., and Dutta, P., Hand book of Zeolite Science and Technology, Marcel Dekker, 2003.
14. Keeting, Ph., Oursler, M., Wiegand, K., Bonde, S., Spelsberg, T., and Riggs, B., “Zeolite A Increases Proliferation, Differentiation, and Transforming Growth Factor β Production in Normal Adult Human Osteoblast-like Cells in Vitro”, Bone and Mineral Reacharch, Vol. 7, p. 11, 1992.
15. Tavolaro, P., Martino, G., Andò, S., and Tavolaro, S., “Zeolite Scaffold for Culture of Human Breast Cancer Cells. Part II: Effect of and Hybrid Zeolite Membranes on Neoplastic and Metastatic Activity Control”, Materials Science and Engineering, Vol. 68, pp. 474-481 , 2018.
16. Ninan, N., Muthunarayanan, M., Nur Aliza Bt, Y., In-Kyu Park, A. E., and Tin, W., “Antibacterial and Wound Healing Analysis of Gelatin Zeolite Scaffolds”, Colloids and Surfaces B: Biointerfaces, Vol. 115, pp. 244-252, 2014.
17. Diba, M., Fathi, M. H., and Kharaziha, M., “Novel Forsterite/Polycaprolactone Nanocomposite Scaffold for Tissue Engineering Applications”, Materials Letters, Vol. 65, pp. 1931-1934, 2011.
18. Ninan, N., Grohens, Y., Elain, A., Kalarikkal, N., and Thomas, S., “Synthesis and Characterization of Gelatin/Zeolite Porous Scaffold”, European Polymer Journal, Vol. 49, pp. 2433-2445, 2013.
19. Akmammedove, R., Huysal, M., Isik, S., and Senel, M., “Preparation and Characterization of Novel Chitosan/Zeolite Scaffolds for Bone Tissue Engineering Applications”, Taylor and Francis, Https:// DOI: 10.1080/00914037.2017.1309539, 2017.
20. Zhu, R., Chen, Y., Ke, Q., Gao, Y., and Guo, Y., “SC79-Loaded ZSM-5/Chitosan Porous Scaffolds With Enhanced Stem Cell Osteogenic Differentiation and Bone Regeneration”, Journal of Materials Chemistry B, Vol. 285, No. 5, pp. 1670-1676, 2017.
21. Diba, M., Kharaziha, M., Fathi, M. H., Gholipourmalekabadi, M., and Samadikuchaksaraei, A., “Preparation and Characterization of Polycaprolactone/Forsterite Nanocomposite”, Composites Science and Technology, Vol. 72, pp. 716-723, 2012.
22. Davarpanah Jazi, R., Rafienia, M., Salehi Rozve, H., Karamian, E., and Sattary, M., “Fabrication and Characterization of Electrospun Poly lactic-co-Glycolic acid/Zeolite Nanocomposite Scaffolds using Bone Tissue Engineering”, Journal of Bioactive and Compatible Polymers, Vol. 33, No. 1, pp. 63-78, 2018.
23. Mallick, S., Tripathi, S., and Srivastava, P., “Advancement in Scaffolds for Bone Tissue Engineering: A Review”, Journal of Pharmacy and Biological Sciences, Vol. 10, No. 1, pp. 37-54, 2015.
24. Lee, E., Teng, S. H., Jang, T., Wang, P., Yook, S., Kim, H., and Koh, Y., “Nanostructured Poly (e-caprolacton)-Silica Xerogel Fibrous Memberan for Guided Bone Regeneration”, Acta Biomaterialia, Vol. 6, pp. 3557-3565, 2010.
25. Fujihara, K., Kotaki, M., and Ramakrishna, S., “Guided Bone Regeneration Memberane Made of Polycaprolactone/Calcium carbonate Composite Nanofibres”, Biomaterials, Vol. 26, pp. 802-805, 2010.
26. Bianco, A., Federico, E., Moscatelli, I., Camaioni, A., Armentano, I., Campagnolo, L., Dottori, M., Kenny, J., Siracusa, G., and Gusmano, G., “Electrospun Poly(caprolactone)/ Ca- deficient Hydroxyapatite Nanohybrids: Miicrostructure, Mechanical Properties and Cell Response by Murine Embryonic ste Cells”, Materials Science and Engineering C, Vol. 29, pp. 2063-2071, 2009.
27. Gaharwar, A., Rivera, Ch., Wu, Ch., and Schmidt, G., “Transparent, Elastomeric and Tough Hydrogels from Poly(ethylene glycol) and Silicate Nanoparticles”, Acta Biomatherialia, Vol. 7, pp. 4139-4148, 2011.
28. Christopher, X. F. L, “Dynamics of In vitro Polymer Degradation of Polycaprolactone-based Scaffolds: Accelerated Versus Simulated Physiological Conditions”, Biomedical Materials, Vol. 3, No. 3, pp. 034108-034108, 2008.
29. Kharaziha, M., Fathi, M. H., Edris, H., “Development of Novel Aligned Nanofibrous Composite Membranes for Guided Bone Regeneration”, Journal of the Mechanical Behavior of Biomedical Materials, Vol. 24, pp. 9-20, 2013.
30. Iqbal, N., “Nanohydroxyapatite Reinforced Zeolite ZSM Composites: A Comprehensive study on the Structural and in vitro Biological Properties”, Ceramics International, Vol. 42, pp. 7175-718, 2016.
Journal of Advanced Materials in Engineering (Esteghlal)
Volume 39, Issue 4
February 2021
Pages 77-94

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