Synthesis and Characterization of Magnetic Hybrid Microgels for Identification of Breast Cancer Cells

Document Type : Original Article

Authors

1 Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran

2 School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, Tehran, Iran

Abstract

The objective of the current study is to synthesize magnetic microgels containing cobalt ferrite nanorods, surface modified with aptamer, for efficient separation and identification of breast cancer cells in the presence of a magnetic field. To construct magnetic microgels, cobalt ferrite nanorods were synthesized at 600 °C with a 5 wt.% of polyvinyl alcohol. The synthesized cobalt ferrite nanorods were successfully coated with chitosan, and then the coated nanorods were loaded into alginate microgels. Magnetic microgel samples were synthesized at different pH levels to achieve the optimal magnetic microgels. The sample synthesized at pH 7 of alginate solution and a final pH of 3 (7alg-3) was chosen due to its better size of the porosity and the percentage of surface porosity. Scanning electron microscopy test was conducted to investigate the morphological, and structural properties of nanorods and magnetic microgels. Finally, the surface of the optimal microgel was modified with mucin 1 aptamer, and visible-UV spectroscopy tests were performed to examine the aptamer attachment to the microgel surface. As a result of these efforts, a magnetic aptasensor capable of detecting MCF7 cancer cells and measuring saturation magnetization was developed. Physical and structural properties of the magnetic aptasensor and its components were investigated using scanning electron microscopy, visible-UV spectroscopy, and vibrating sample magnetometry tests. The detection limit and sensitivity of the aptasensor were measured, resulting in 14 cells/mL and a sensitivity of 0.18 Ms/cell.

Keywords

Main Subjects

References

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Journal of Advanced Materials in Engineering (Esteghlal)
Volume 43, Issue 1
June 2024
Pages 1-11

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