A Novel Fibrous Polycaprolactone/Gelatin Wound Dressing Containing Vitamin C and Antibacterial Drug For Wound Healing

Document Type : Original Article

Authors

1 Department of Tissue Engineering, Islamic Azad University, Najaf Abad branch,Najafabad, Iran

2 2- Department of Biomaterials, Nanotechnology and Tissue Engineering, Faculty of Modern Medical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 3- Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Biochemistry, Islamic Azad University, Najaf Abad branch, Najafabad, Iran

Abstract

Skin wounds caused by burns, physical injuries, and diseases such as diabetes can lead to amputation and even death of people due to infection and lack of proper angiogenesis. In this research, a wound dressing was made using the electrospinning method from a blend of poly-caprolactone and gelatin polymers which Ciprofloxacin and Vitamin C were loaded in the polymers separately. The morphology of the structure was observed by scanning electron microscope followed by evaluation of hydrophilicity, swelling, degradation rate, tensile strength, and drug release pattern. Fibroblast cell response was also considered using cytotoxicity test method. Results showed that the wound dressing fibers without drugs and containing drugs were uniform with a diameter of 1039.246 ± 96 and 1403± 406 nm, respectively. The wound dressing showed a suitable wide range of swelling ability and durability within 35 days. The tensile strength increased up to 3.5times by addition of ciprofloxacin and vitamin C due to the morphological changes. The presence of drugs in the system did not indicate any adverse effect on the cell's viability, and suitable proliferation and  adhesion of skin fibroblast cells were observed on the surface of the sample.  In addition,  a combination  of gelatin and polycaprolactone polymers could provide an appropriate degradation rate and release pattern without any cross-linking process, while preserving the mechanical properties and flexibility.

Keywords

Main Subjects

References

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Journal of Advanced Materials in Engineering (Esteghlal)
Volume 43, Issue 1
June 2024
Pages 13-26

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