Study of the Biological Properties of 68S Bioactive Glass Stabilized at Temperatures of 600-800 °C to Improve its Biocompatibility in Bone Tissue Engineering Therapeutic Applications

Document Type : Original Article

Authors

Department of Materials Engineering, Imam Khomeini International University, Qazvin, Iran

Abstract

In this research, 68S bioactive glass contaminated with strontium was synthesized at stabilization temperatures of 600-800 °C by sol-gel method, and the effect of stabilization temperature on the in vitro bioactive properties, before and after 14 days of immersion in Simulated body Fluid was investigated by characterization tests and bioassays. According to the results of thermal tests, the weight loss of the sample up to 620 °C was observed due to the removal of nitrate from the bioactive glass structure and the stability of its weight loss at 700 °C. The results of X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy indicated the formation of hydroxyapatite layer on the 3rd and 5th day of immersion and their height increase until the 14th day of immersion for 68S7C and 68S8C samples, respectively. Also, according to the results of the Inductively Coupled Plasma test, the higher formation rate of hydroxyapatite layer on the surface of the 68S7C sample compared to the 68S8C sample was confirmed. In addition, according to the results of cytotoxicity and alkaline phosphatase tests, the growth and cell proliferation of MC3T3-E1 osteogenic cells in the 68S7C sample compared to the 68S8C sample improved by 13% and 7%, respectively. Therefore, according to the results of the tests, the 68S7C sample due to its stabilization at the optimal temperature of 700 °C, the controlled rate of destruction of the glass network, having a higher formation rate of hydroxyapatite layer, improving the growth and cell proliferation of MC3T3-E1 bone-forming cells and consequently improving its biocompatibility, was selected and introduced as a reliable option for bone tissue engineering therapeutic applications.

Keywords

Main Subjects

References

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Journal of Advanced Materials in Engineering (Esteghlal)
Volume 43, Issue 1
June 2024
Pages 67-80

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