Study of the Biological Properties of 68S Bioactive Glass Stabilized at Temperatures of 600-800 °C to Improve its Biocompatibility in Bone Tissue Engineering Therapeutic Applications

Document Type : Original Article

Authors

Department of Materials Engineering, Imam Khomeini International University, Qazvin, Iran

Abstract

In this research, 68S bioactive glass contaminated with strontium was synthesized at stabilization temperatures of 600-800 °C by sol-gel method, and the effect of stabilization temperature on the in vitro bioactive properties, before and after 14 days of immersion in Simulated body Fluid was investigated by characterization tests and bioassays. According to the results of thermal tests, the weight loss of the sample up to 620 °C was observed due to the removal of nitrate from the bioactive glass structure and the stability of its weight loss at 700 °C. The results of X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy indicated the formation of hydroxyapatite layer on the 3rd and 5th day of immersion and their height increase until the 14th day of immersion for 68S7C and 68S8C samples, respectively. Also, according to the results of the Inductively Coupled Plasma test, the higher formation rate of hydroxyapatite layer on the surface of the 68S7C sample compared to the 68S8C sample was confirmed. In addition, according to the results of cytotoxicity and alkaline phosphatase tests, the growth and cell proliferation of MC3T3-E1 osteogenic cells in the 68S7C sample compared to the 68S8C sample improved by 13% and 7%, respectively. Therefore, according to the results of the tests, the 68S7C sample due to its stabilization at the optimal temperature of 700 °C, the controlled rate of destruction of the glass network, having a higher formation rate of hydroxyapatite layer, improving the growth and cell proliferation of MC3T3-E1 bone-forming cells and consequently improving its biocompatibility, was selected and introduced as a reliable option for bone tissue engineering therapeutic applications.

Keywords

Main Subjects

References

  1. Anand A, Kankova H, Hajovska Z, Galusek D, Boccaccini A, Galuskova D. Bio-response of copper–magnesium co-substituted mesoporous bioactive glass for bone tissue regeneration. Mater Chem B. 2024;12 (1):1875-1891. https://doi.org/10.1039/D3TB01568H.
  2. Fendi F, Abdullah B, Suryani S, Raya I, Tahir D, Iswahyudi I. Hydroxyapatite based for bone tissue engineering: innovation and new insights in 3D printing technology. Polym Bull. 2024; 81(1):1097–1116. https://doi.org/10.1007/s00289-023-04794-6.
  3. Baghban eslaminezhad MR, Nejati E, Zandi M, Zomorodian E, Bagheri F. Study of mesenchymal stem cell proliferation and bone differentiation on composite scaffolds of PLLA and Nano Hydroxyapatite with different morphologies. Cell J. 2011; 122(12):469-476. https://sid.ir/paper/556932/en.
  4. Negut I, Ristoscu C. Bioactive Glasses for Soft and Hard Tissue Healing Applications-A Short Review. Appl Sci. 2023; 13(10):6151. https://doi.org/10.3390/ app13106151.
  5. Nishikawa T, Kokubu M, Kato H, Imai K, Tanaka A. Confocal laser scanning microscopy in study of bone calcification. Appl Surf Sci. 2012; 262(82):64-68. https://doi.org/10.1016/j.apsusc.2012.01.163.
  6. Hench L. Bioceramics: from concept to clinic. Am Ceram Soc. 1991; 74(18):1487-1510. https://doi.org/ 10.1111/j.1151-2916.1991.tb07132.x.
  7. Vafa E, Tayebi E, Abbasi M, Azizli MJ, Bazargan-Lari R, Talaiekhozani A. A better roadmap for designing novel bioactive glasses: effective approaches for the development of innovative revolutionary bioglasses for future biomedical applications. Environ Sci Pollut Res. 2023; 30(1): 116960–116983. https://doi.org/10.1007/s11356-022-24176-1.
  8. Dziadek M, Dziadek K, Checinska K, Zagrajczuk B, Cholewa-Kowalska K. Bioactive Glasses Modulate Anticancer Activity and Other Polyphenol-Related Properties of Polyphenol-Loaded PCL/ Bioactive Glass Composites. ACS Appl Mater Interfaces. 2024; 16(19):24261–24273. https://doi.org/10.1021/acsami. 4c02418.
  9. Balbinot GS, Leitune VGB, Bahlis EAC, Ponzoni D, Visioli F, Collares FM. Niobium-containing bioactive glasses modulate alkaline phosphatase activity during bone repair. J Biomed Mater Res B Appl Biomater. 2023; 111(6):1224-1231. https://doi. org/10.1002/jbm.b.35227.
  10. Deliormanl A, Yıldırım M. Sol-gel synthesis of 1393 bioactive glass powders containing therapeutic agents. J Aust Ceram Soc. 2016; 52(21):9-19. https:// aperta.ulakbim.gov.tr/record/57399.
  11. Ghaebi Panah N, Atkin R, Timothy B. Effect of low temperature crystallization on 58S bioactive glass sintering and compressive strength. Ceram Int. 2021; 47(21):30349-30357. https://doi.org/10.1016/j.ceramint. 2021.07.215.
  12. Dang T, Bui T, Guseva E, Ta A, Nguyen A, Hoang T. Characterization of bioactive glass synthesized by sol-gel process in hot water. Cryst. 2020; 10(2):529-541. https://doi.org/10.3390/cryst10060529.
  13. Shivalingam Ch, Purushothaman B, Dhinasekaran D, Padmanaban R, Subramanian B. Role of sintering temperature dependent crystallization of bioactive glasses on erythrocyte and cytocompatibility. Process Appl Ceram. 2019; 13(1):12-23. http://dx.doi.org/10. 2298/PAC1901012C.
  14. Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solutions able to reproducein vivo surface-structure changes in bioactive glass-ceramic A-W3. J Biomed Mater Res. 1990; 24(6):721–734. https://doi.org/10.1002/jbm.820240607.
  15. Zhao R, Wang Z, Gu L, Ma Z, Zheng H, Wang Q. Unraveling the relationship between the structural features and solubility properties in Sr-containing bioactive glasses. Ceram Int. 2024; 50(3):4245-4255. https://doi.org/10.1016/j.ceramint.2023. 07.240.
  16. Ma J, Chen C, Wang D, Hu J. Synthesis, characterization and in vitro bioactivity of magnesium-doped sol–gel glass and glass-ceramics. Ceram. 2011; 37(5):1637–1644. https://doi.org/10.1016/j.ceramint. 2011. 01.043.
  17. Hesaraki S, Alizadeh M, Nazarian H, Sharifi D. Physico-chemical and in vitro biological evaluation of strontium/calcium silicophosphate glass. Mater Sci. 2010; 21(2):695–705. https://doi.org/10.1007 /s10856-009-3920-0.
  18. Hesaraki S, Gholami M, Vazehrad S, Shahrabi S. The effect of Sr concentration on bioactivity and biocompatibility of sol–gel derived glasses based on SiO2–P2O5–CaO–SrO quaternary system. Mater Sci Eng C. 2010; 30(3):383-390. https://doi.org/10.1016/ j.msec.2009.12.001.
  19. Gupta N, Santhiya D, Aditya A, Badra K. Dendrimer templated bioactive glass-ceramic nanovehicle for gene delivery applications. RSC Adv. 2015; 5(70):56794-56807. https://doi.org/10.1039/C5RA04441C.
  20. Chen X, Guo C, Zhao N. Preparation and characterization of the sol-gel nano-bioactive glasses modified by the coupling agent gamma-aminopropyltriethoxysilane. Appl Surf Sci. 2008; 255(2):466–468. https://doi. org/10.1016/j.apsusc.2008. 06.061.
  21. Veres R, Vanea E, Gruian C, Baia L, Simon V. The effects of PEG assisted synthesis and zinc addition on gamma irradiated bioactive glasses. Compos B Eng. 2014; 66(1): 83-88. https://doi.org/10.1016/j. compositesb.2014.05.006.
  22. Chen J, Ma C, Wang D, Hu J. Effect of magnesia on structure, degradability and in vitro bioactivity of SiO2–CaO–MgO–P2O5 system ceramics. Mater Lett. 2011; 65(1):130–133. https://doi.org/10.1016/ j.matlet. 2010.09.040.
  23. Ereiba K, Abd Raboh A, Mostafa A. Characterization of some bioactive glasses based on SiO2–CaO–P2O5–SrO quaternary system prepared by sol–gel method. Nat Sci. 2014;12(5):97-105. http://dx.doi.org/10.1080/15533174. 783869.
  24. Wu X, Meng G, Wang S, Wu F, Huang W, Gu Z. Zn and Sr incorporated 64S bioglasses: material characterization, in-vitro bioactivity and mesenchymal stem cell responses. Mater Sci Eng C. 2015; 52(1):242–250. https://doi.org/10.1016/j.msec.2015.03.057.
  25. Sharifianjazi F, Parvin N, Tahriri M. Formation of Apatite Nano-Needles on Novel Gel Derived SiO2-P2O5-CaO-SrO-Ag2O Bioactive Glasses. Ceram Int. 2017; 43(17): 15214-15220. https://doi.org/10.1016/j. ceramint.2017.08.056.
  26. Liu J, Rawlinson S, Hill R, Fortune F. Strontium-substituted bioactive glasses in vitro osteogenic and antibacterial effects. Dent Mater. 2016; 32(3):412–422. https://doi.org/10.1016/j.dental.2015.12.013.
  27. Isaac J. Effects of strontium-doped bioactive glass on the differentiation of cultured osteogenic cells. Eur Cell. 2011; 21(1473-2262): 130–14307. https://doi. org/22203/ecm.v021a11. PMID: 21305476.
  28. Jiménez M, Abradelo C, San Román J, Rojo L. Bibliographic review on the state of the art of strontium and zinc based regenerative therapies. Recent developments and clinical applications. J Mater Chem B. 2019; 7(12):1974–1985. https://doi. org/10.1039/C8TB02738B.
  29. Sourav D, Bavya D, Sanjay G, Biswanath K, Vamsi K, Mangal R. Mechanical and in Vitro Degradation Behavior of Magnesium‐bioactive Glass Composites Prepared by SPS for Biomedical Applications. J Biomed Mater Res B Appl Biomater. 2019; 107(2):352–65. https://doi.org/10.1002/jbm.b.34127.
  30. Tabiaa Z, El Mabrouk Kh, Brichaa M, Nouneh Kh. Mesoporous Bioactive Glass Nanoparticles Doped with Magnesium: Drug Delivery and Acellular in Vitro Bioactivity. RSC Adv. 2019; 9(22):12232-12246. https://doi.org/10.1039/C9RA01133A.
  31. Nawaz Q, Pablos-Martin A, Souza e Silva JM, Hurle K, Jaimes A, Brauer D, Boccaccini A. New insights into the crystallization process of sol-gel–derived 45S5 bioactive glass. Am Ceram Soc. 2020; 103 (8):4234-4247. https://doi.org/10.1111/jace.17124.
  32. Fandzloch M, Bodylska W, Augustyniak AW, Roszek K, Jaromin A, Lukowiak A. Bioactive nanoglasses and xerogels (SiO2–CaO and SiO2–CaO–P2O5) as promising candidates for biomedical applications. Ceram Int. 2023; 49(5):7438-51. https:// doi.org/10.1016/j.ceramint.2022.10. 225.
  33. Luz G, Mano J. Preparation and characterization of bioactive glass nanoparticles prepared by sol–gel for biomedical applications. Nanotechnol. 2011;22(49):344-417. https://doi.org/10.1088/0957-4484/22/49/494014.
  34. Thomas A, Bera J. Sol–gel synthesis and in vitro bioactivity of glass–ceramics in SiO2–CaO–Na2O–P2O5 J Sol-Gel Sci Technol. 2016; 80(2): 411-416. https://doi.org/10.1007/s10971-016-4110-4.

 

 

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