Fabrication and Characterization of Novel Willemite Nanobioceramic for Bone Defect Repair

Authors

Abstract

The positive effect of Si and Zn ions on bone formation and metabolism has already been confirmed. The aim of this study was preparation and characterization of Willemite (Zn2SiO4) for the repair of bone defects. Willemite was prepared through solid state reaction. Phase analysis and chemical compositions were investigated. The zeta potential of the nanoparticles was determined in physiological saline, and compressive strength and Young's modulus of the samples were measured. The ability of hydroxyapatite formation was investigated in simulated body fluid (SBF) and cytotoxicity of the particles was evaluated in contact with human bone marrow stem cells. The results of this study showed that Willemite nanobioceramic is obtained with the expected chemical composition and negative zeta potential. The results also showed that the hydroxyapatite forming ability in SBF was not strong. MTT assay confirmed the cell proliferation and availability in contact with a specific concentration of Willemite nanoparticles. All these findings indicate that Willemite nanobioceramic with proper biocompatibility can be suggested as a novel biomaterial for the repair of bone defects.

Keywords


Kim, J., Baek, S., Lee, S.H., Park, E.K., Kim, K.C.,
Kwun, I.S. and Shin, H.I., "Zinc-Deficient Diet
Decreases Fetal Long Bone Growth through
Decreased Bone Matrix Formation in Mice", Journal
of Medical Food, Vol. 12, pp. 118-123, 2009. 2. Carlisle, E.M., "Silicon: A Possible Factor in Bone
Calcification", Science, Vol. 167, pp. 279-280, 1970. 3. Carlisle, E.M., "Silicon: An Essential Element for
Chick", Nutrition Reviews, Vol. 40, pp. 210-213, 1982. 4. Doostmohammadi, A., Monshi, A., Fathi, M.H. and
Daniels, A.U., "A Comparative Physico-Chemical
Study of Bioactiveglass and Bone-Derived
Hydroxyapatite", Ceramics International, Vol. 37, pp. 1601–1607, 2011
5. Parisi, A.F. and Vallee, B.L., "Zinc Metalloenzymes:
Characteristics and Significance in Biology and
Medicine", Americal Journal of Clinical Nutrition, Vol 22, pp. 1222-1239, 1969. 6. Hsieh, H.S. and Navia, J.M., "Zinc Deficiency and
Bone Formation Inguinea-Pigs Alveolar Implants",Journal of Nutrition, Vol. 8, pp. 1581-1588, 1980.
7. Yamaguchi, M., “Role of Zinc in Bone Formation
Resorption”, Journal of Trace Elements in
Experimental Medicine, Vol. 11, pp. 119-135, 1998. 8. Caplan, A.L., “Adult Mesenchymal Stem Cells for
Tissue Engineering Versus Regenerative Medicine”,
Journal of Cell Physiology, Vol. 213, pp. 341-347, 2007. 9. Zhang, M., Zhai, W. and Chang, J., “Preparation and
Characterization of a Novel Willemite Bioceramic”,
Journal of Materials Science: Materials in Medicine, Vol. 21, pp. 1169-1173, 2010. 10. Guo, Y.P., Ohsato, H. and Kakimoto, K.I.,
“Characterization and Dielectric Behavior of
Willemite and TiO2-Doped Willemite Ceramics at
Millimeter-Wave Frequency”, Journal of the
European Ceramic Society, Vol. 26, pp. 1827-1832, 2006. 11.Bagambisa, F.B., Joos, U. and Schilli, W.,
“Mechanisms and Structure of the Bond Between
Bone and Hydroxyapatite”, Journal of Biomedical
Materials Research, Vol. 27, pp. 1047–1055, 1993. 12. Oppermann, D.A., Crimp, M.J. and Bement, D.M.,
“In Vitro Stability Predictions for the
Bone/Hydroxyapatite Composite System”, Journal of
Biomedical Materials Research, Vol. 42, pp. 412- 416, 1998. 13.Balamurugan, A., Balossier, G., Kannan, S., Michel,
J., Rebelo, A. and Ferreira, J., "Development and In
Vitro Characterization of Sol-Gel Derived CaO– P2O5–SiO2–ZnO Bioglass", Acta Biomaterialia, Vol. 3, pp. 255–262, 2007. 14. Doostmohammadi A., Monshi, A., Salehi, R., Fathi,
M.H., Golnia, Z. and Daniels, A.U., “Bioactive Glass
Nanoparticles with Negative Zeta Potential”,
Ceramics International, Vol. 37, pp. 2311-2316, 2011. 15. Smeets, R., Kolk, A., Gerressen, M., Driemel, O.,
Maciejewski, O., Hermanns-Sachweh, B., Riediger,
D. and Stein, J.M., “A New Biphasic Osteoinductive
Calcium Composite Material with a Negative Zeta
Potential for Bone Augmentation”, Head Face
Medicine, Vol. 13, pp. 5-13, 2009. 16.Cooper, J.J. and Hunt, J.A., “The Significance of
Zeta Potential in Osteogenesis, Society for
Biomaterials”, Proceedings of Transactions of the
31st Annual Meeting for Biomaterials, London,
pp. 33-592, 2006. 17. Doostmohammadi A., Monshi, A., Salehi, R., Fathi,
M.H., Karbasi, S., Pieles, U. and Daniels, A.U., “Preparation, Chemistry and Physical Properties of
Bone-Derived Hydroxyapatite Particles Having a
Negative Zeta Potential”, Materials Chemistry and
Physics, Vol. 132, pp. 446-452, 2012. 18. Teng, N.C., Nakamura, S., Takagi, Y., Yamashita,
Y., Ohgaki, M. and Yamashita, K., “A New
Approach to Enhancement of Bone Formation by
Electrically Polarized Hydroxyapatite”, Journal of
Dental Research, Vol. 80, pp. 1925-1929, 2001. 19. Katherine, R., Evans, P. and Jason, P.C., “Feasibility
of a Braided Composite for Orthopedic Bone Cast”,
The Open Biomedical Engineering Journal, Vol. 7, pp. 9-17, 2013. 20. Zargar Kharazi, A., Fathi, M.H. and Bahmany, F.,
“Design of a Textile Composite Bone Plate Using
3D-Finite Element Method”, Materials Design, Vol. 31, pp. 1468–1474, 2010. 21. Huang, Z. and Fujihara, K., “Stiffness and Strength
Design of Composite Bone Plates”, Composite
Science Technology, Vol. 65, pp. 73-85, 2005. 22.Reffitt D.M., Meenan, N.J., Sanderson, J.D.,
Jugdaohsingh, R., Powell, J.J. and Thompson, R.P.,
“Bone Density Improves with Disease Remission in
Patients with Inflammatory Bowel Disease”,
European Journal Gastroenterol Hepatol, Vol. 15, pp. 1267-1273, 2003.. 23. Xynos, I.D., Edgar, A.J., Buttery, L.D., Hench L.L.
and Polak, J.M., “Ionic Products of Bioactive Glass
Dissolution Increase Proliferation of Human
Osteoblasts and Induce Insulin-Like Growth Factor II
mRNA Expression and Protein Synthesis”,
Biochemical and Biophysical Research
Communications, Vol. 276, pp. 461-465, 2000. 24. Wu, C. and Chang, J., “Degradation, Bioactivity, and
Cytocompatibility of Diopside, Akermanite, and
Bredigite Ceramics”, Journal of Biomedical
Materials Research Part B: Applied Biomaterials, Vol. 83, pp. 153-160, 2007.

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