داربست نانولیفی پلی هیدروکسی بوتیرات کوهیدروکسی والرات حاوی نانوذرات هیدروکسی آپاتیت/ بریدیجیت: مشخصه‌یابی و ارزیابی بیولوژیکی

نویسندگان

1 1. گروه بیومواد، دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان

2 2. مرکز نانوالیاف و نانوتکنوژی، دانشکده مهندسی مکانیک، دانشگاه ملی سنگاپور، سنگاپور

چکیده

در این تحقیق، داربست نانولیفی کامپوزیتی پلی هیدروکسی بوتیرات کوهیدروکسی والرات (PHBV) حاوی نانوذرات کامپوزیتی هیدروکسی آپاتیت/بریدیجیت (HABR) به روش الکتروریسی تولید شد. مورفولوژی نانوالیاف تولید شده و نحوه توزیع نانوذرات در نانوالیاف به‌ترتیب توسط میکروسکوپ الکترونی روبشی و عبوری بررسی شدند. ارزیابی خواص مکانیکی نشان داد که یک حد آستانه‌ای برای غلظت نانوذرات وجود دارد که در آن، حضور نانوذرات باعث بهبود خواص مکانیکی داربست نانولیفی شد. داربست کامپوزیتی PHBV خاصیت ترشوندگی بهتری در مقایسه با PHBV خالص از خود نشان داد. در آزمون کشت سلول برون‌تنی، سلول‌های استئوبلاست (hFob) بر داربست‌های نانولیفی کشت داده شدند.ارزیابی تکثیر سلولی به‌روش MTS نشان داد که بعد از 10 و 15 روز، سلول‌ها بر داربست کامپوزیتی PHBV/HABR به‌طور معنی‌داری بیشتر از داربست خالص PHBV رشد داشته‌اند. به‌علاوه نتایج آنالیز ارزیابی میکروسکوپی الکترونی روبشی- طیف‌سنجی تفریق انرژی و رنگ آمیزی سلولی نشان دادند که سلول‌های کشت داده شده برروی داربست کامپوزیتی PHBV بیشتر از داربست PHBV خالص و نمونه کنترل، رسوبات مینراله تشکیل دادند. نتایج این مطالعه نشان داد که نانوالیاف کامپوزیتی PHBV/HABR با خواص مکانیکی، ترشوندگی و رفتار سلولی بهبود یافته، پتانسیل خوبی در کاربردهای بازسازی بافت استخوان دارند.
 

کلیدواژه‌ها

عنوان مقاله [English]

Poly (hydroxybutyrate co hydroxyvalerate) Nanofibrous Scaffold Containing HydroxyapatiteBredigite Nanoparticles: Characterization and Biological Evaluation

نویسندگان [English]

  • M. Kouhi 1
  • M. Shamanian 1
  • M. Fathi 1
  • Molamma Prabhakaran 2
  • Seeram Ramakrishna 2
1 1. Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.
2 2. Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, National University of Singapore, Singapore.
چکیده [English]

In this work, poly (hydroxybutyrate co hydroxyvalerate) (PHBV) composite nanofibrous scaffold containing hydroxyapatite/bredigite (HABR) nanoparticles was fabricated through electrospining method. The morphology of prepared  nanofibers and the state of the nanoparticles dispersion in nanofiber matrix were investigated using scanning and transmission electron microscopy, respectively. Evaluation of the mechanical properties of the nanofibrous scaffolds revealed that there is a limit to the nanoparticle concentration at which nanoparticles can improve the mechanical properties of the nanofibrous scaffolds. According to the results, PHBV/HABR nanofibers showed higher wettability compared to PHBV nanofibers. In vitro cell culture assay was done using human fetal osteoblast cells on nanofibrous scaffold. MTS assay revealed that cell proliferation on the composite nanofibrous scaffold was significantly higher than those on the pure scaffold after 10 and 15 days. Scanning electron microscopy- Energy dispersive X-ray spectroscopy and CMFDA colorimeter assay analysis showed that the cells on the PHBV/HABR scaffolds acquired higher mineral deposition than the cells on the pure PHBV and control sample scaffold. Based on the results we concluded that PHBV/HABR nanofibers scaffold with higher wettability, improved mechanical properties and cell behavior hold great potential in bone regeneration applications.

کلیدواژه‌ها [English]

  • Nanofibrous scaffold
  • Poly (hydroxybutirate co hydroxyvalerate)
  • hydroxyapatite
  • Bredigite
  • Bone regeneration

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مواد پیشرفته در مهندسی
دوره 36، شماره 3
آبان 1396
صفحه 87-99

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