اصلاح ابرآبدوست و آنتی باکتریال پلیمر پلی‌اورتان به‌کمک نانوذرات دی‌اکسید تیتانیوم به‌منظور استفاده در ابزار پزشکی

نویسندگان

دانشکده علوم و فناوری های نوین، دانشگاه اصفهان

چکیده

پلیمر پلی­‌اورتان نقش مهمی در مراقبت‌­های بهداشتی دارد و به‌طور گسترده در دستگاه­‌ها و ابزارهای پزشکی مورد استفاده قرار می­گیرد. با این حال، هنوز زیست­سازگاری پایین و تشکیل بیوفیلم در سطح آن، موضوعی چالش برانگیز است. اصلاح ترشوندگی سطح روشی مؤثر برای افزایش زیست­سازگاری سطح پلیمر­های دارای خواص توده مطلوب است. در این مطالعه، اصلاح سطح صفحات پلی­اورتان توسط لایه نازک پلی­اتیلن گلیکول و به دام افتادن نانوذرات دی‌اکسید تیتانیوم در پلیمر به‌کمک تغییر ساختار فیزیکی و شیمیایی سطح به‌منظور افزایش زیست­سازگاری انجام گرفت. خواص فیزیکوشیمیایی سطوح اصلاح شده با استفاده از طیف‌سنجی تبدیل فوریه مادون قرمز (FTIR)، میکروسکوپ الکترونی روبشی (SEM)، آنالیز پراش انرژی پرتو ایکس(EDX) و اندازه‌گیری زاویه تماس و انرژی آزاد سطح مشخص شد. زیست­سازگاری سطوح اصلاح شده با استفاده از آزمون سمیت MTT بر سلول­های سرطانی دهانه رحم، چسبندگی باکتریایی­‌ها و بررسی تشکیل بیوفیلم و سنجش جذب پروتئین به‌روش برادفورد مورد بررسی قرار گرفت. یک لایه نازک قابل اتوکلاو و ارزان با ساختار دارای زبری چندگانه و پایدار به‌طور کووالان روی سطح صفحات پلی­اورتان برای کاربردهای زیستی و پزشکی ایجاد شد. نتایج نشان می­دهد که به‌غیر از فعالیت‌­های ضد باکتریایی، نمونه اصلاح شده نیز توانایی کاهش تشکیل بیوفیلم را دارد به‌طوری‌که حداکثر مهار بیوفیلم در 24 ساعت اول 94 درصد بیشتر از نمونه اصلاح نشده است.

کلیدواژه‌ها


عنوان مقاله [English]

Modification of Superhydrophilic, Anti-bacterial Polyurethane Polymer with TiO2 Nanoparticles for Medical Devices

نویسندگان [English]

  • A. Razmjou
  • F. Noorisafa
  • N. Emami
Department Advance Sciences and Technology, Isfahan University, Isfahan, Iran.
چکیده [English]

Polyurethane polymer plays an important role in health care, and it is widely used in medical devices and instruments. However, the low biocompatibility and biofilm formation on the surface can be regarded as a challenging issue. Engineering the wetting capability of the surface is an effective way to increase the biodegradability of polymer surfaces with sufficient bulk properties. In this study, the surface modification of polyurethane sheets by a thin layer of polyethylene glycol and trapping of titanium dioxide nanoparticles were carried out by means of physical and chemical changes on the surface to enhance the biocompatibility. The physicochemical properties of the modified surfaces were determined using fourier-transform infrared (FTIR) spectroscopy , scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and contact angle and free surface energy measurement. The biocompatibility of the modified levels was evaluated using the MTT toxicity test on cervical cancer cells (HeLA), bacterial adhesion, biofilm formation, and the protein absorption assay by the Bradford method. A thin, autoclave able and inexpensive thin layer with a solid and stable roughening structure was created covalently on the surface of the polyurethane plates for biological and medical applications. The results, therefore, showed that apart from antibacterial activity, the modified sample also had the ability to reduce the biofilm formation, such that the maximum biofilm attachment inhibition in the first 24 hours was 94% higher than that of the modified sample.

کلیدواژه‌ها [English]

  • Biomaterial
  • Ssuperhydrophilic
  • TiO2 nanoparticles
  • Polyethylene glycol
  • polyurethane
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