بررسی اثر افزودن نانورس بر خصوصیات داربست نانوکامپوزیتی پلی‌کاپرولاکتون حاوی سلول‌های بنیادی مزانشیمی مشتق از بافت چربی به‌منظور کاربرد در مهندسی بافت نرم

نویسندگان

پژوهشکده زیست‌فناوری پزشکی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران

چکیده

داربست‌های مهندسی بافت، چارچوب‌های زیستی هستند که از رشد، تکثیر و تمایز سلول‌ها در بدن حمایت می‌کنند. در این میان، داربست‌های نانولیفی به‌شکل مناسبی از لحاظ مکانیکی و زیستی از زمینه خارج سلولی تقلید می‌کنند. این داربست‌ها نقش مؤثری در بازسازی و ترمیم بافت ایفا می‌کند. یکی از روش‌های تهیه داربست‌های نانولیفی با خواص دستکاری شده، افزودن نانوذرات به زمینه پلیمری ( نانوکامپوزیت) است. در این پژوهش، الیاف یک‌دست از جنس پلی‌کاپرولاکتون تقویت شده با نانورس هیدروکسید دوگانه لایه‌ای با درصدهای 0/1 درصد تا 10 درصد وزنی توسط روش الکتروریسی تهیه شد. افزودن فاز نانورس به فاز پلیمری باعث کاهش قطر الیاف و بهبود خواص مکانیکی شد. به‌علاوه، حضور نانوذرات رسی در بستر پلی‌کاپرولاکتون به‌شکل قابل توجهی موجب افزایش چسبندگی سلول‌ها و تمایز سلول‌های چربی شد. نتایج نشان می‌دهد می‌توان از داربست‌های الکتروریسی شده پلی‌کاپرولاکتون تقویت شده با نانوذرات رسی در کاربردهای مهندسی بافت نرم استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Nanoclay Addition on the Properties of Polycaprolactone Nanocomposite Scaffolds Containing Adipose Derived Mesenchymal Stem Cells used in Soft Tissue Engineering

نویسندگان [English]

  • S. S. Shafiei
  • M. Shavandi
  • Y. Nickakhtar
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
چکیده [English]

Tissue-engineering scaffolds provide biological and mechanical frameworks for cell adhesion, growth, and differentiation. Nanofibrous scaffolds mimic the native extracellular matrix (ECM) and play a significant role in formation and remodeling of tissues and/or organs . One way to mimic the desired properties of fibrous ECM is adding nanoparticles into the polymer matrix. In the current study, the uniform fibers of poly (ε-caprolactone) (PCL) enriched with different layered double hydroxide (LDH) contents (ranging from 0.1 wt.% to 10 wt.%) were successfully fabricated by electrospinning method. The LDH nano particles were randomly dispersed in the fibers, as confirmed by Energy Dispersive X-ray analysis (EDX). Scaffolds were analyzed from morphological, physical and mechanical view. Biological assessments of scaffolds in terms of cellular attachment and adipogenic differentiation of mouse adipose derived stem cells (mADSCs) were performed. The results showed that inclusion of LDH nanoparticles reduced the average fiber diameter and enhanced the tensile strength and elongation at break values of the PCL scaffold. The LDH-enriched electrospun PCL scaffolds had remarkable effects on cell adhesion. Moreover, a significant increase in adipogenic differentiation of mADSCs was observed. The PCL/LDH nanofibrous scaffolds showed great potential in application for soft tissue engineering.

کلیدواژه‌ها [English]

  • Tissue engineering
  • Soft tissue engineering
  • Scaffold
  • electrospinning
  • Polycaprolactone
  • Layered double hydroxide

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مواد پیشرفته در مهندسی
دوره 39، شماره 4
اسفند 1399
صفحه 45-59

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