ساخت و مشخصه‌یابی نانوکامپوزیت پلی‌کاپرولاکتون- زئولیت Y برای مهندسی بافت استخوان

نویسندگان

1 دانشکده مهندسی مواد و متالورژی دانشگاه علم و صنعت ایران

2 دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، ایران

چکیده

در سال‌های اخیر استفاده از داربست‌های نانوکامپوزیتی پلیمر- سرامیک در مهندسی بافت استخوان به‌دلیل شباهت این ساختارها به بافت طبیعـی اسـتخوان، مورد توجه قرار گرفته است. در این میان، پلی‌کاپرولاکتون در ساخت داربست‌های استخوانی مورد توجه است. کامپوزیت کردن پلی‌کاپرولاکتون با فازهای سرامیکی مانند زئولیت که توانایی بهبود تشکیل استخوان را دارند می‌تواند منجر به بهبود کارایی این پلیمر در داربست‌های استخوانی شود. هـدف از ایـن پـژوهش، سـاخت داربسـت نانوکامپوزیتی پلی‌کاپرولاکتون - زئولیت با خواص مکانیکی، زیست تخریب‌پذیری و زیست فعالی مناسب بـرای کـاربرد در مهندسـی بافـت استخوان اسفنجی است. برای ساخت این داربست از دو روش ریخته‌گری حلال – شستشو ذرات و خشک کردن انجمادی در کنار هم استفاده شد. بررسـی‌هـای میکروسکوپی نشان داد که انـدازه تخلخـل‌هـای داربست‌های حاصل بـین 200 تـا 400 میکرومتر است. نقشه توزیع عنصری، توزیع یکنواخت فاز نانوزئولیت را در زمینه پلی‌کاپرولاکتون تأیید کرد. همچنین با توجه به نتایج طیف‌سنجی مادون قرمز با تبدیل فوریه نوع اتصال نانوذرات زئولیت به زمینه پلی­کاپرولاکتون اتصال فیزیکی تعیین شد. نتایج بررسی خواص مکانیکی داربست‌ها نشان‌دهنده افزایش مدول یانگ و استحکام فشاری (به ترتیب از 0/04 تا 0/3 و 3 تا 7 مگاپاسکال) بعد از اضافه شدن فاز نانوزئولیت به داربست‌ها بـود. با افزودن نانوزئولیت آبدوستی پلی‌کاپرولاکتون افزایش یافت و کاهش وزن بیشتری مشاهده شد (برای داربست حاوی 20 درصد زئولیت 1/6 ± 53/52 درصد)، همچنین تشـکیل هیدروکسـی آپاتیـت در محـیط شبیه‌سازی شده بدن سرعت گرفت. نتایج نشان می‌دهد که داربست‌های ساخته شده قابلیت کاربرد در مهندسی بافت استخوان اسفنجی را دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Fabrication and Characterization of Polycaprolactone – Zeolite Y Nanocomposite for Bone Tissue Engineering

نویسندگان [English]

  • N. Zakeri 1
  • H.R. Rezaie 1
  • J. Javadpour 1
  • M. Kharaziha 2
  • M. Kharaziha 2
1 Department of Materials and Metallurgical Engineering, Iran University of Science and Technology, Narmak, Tehran 16846-13114, Iran.
2 Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
چکیده [English]

In recent years, nanoceramics have been used in scaffolds to emulate the nanocomposite with a three-dimensional structure of natural bone tissue. In this regard, polycaprolactone biopolymer is widely used as a scaffold in bone tissue engineering. The goal of this research is to produce porous scaffolds of polycaprolactone - zeolite biocomposite with suitable mechanical, bioactive and biological properties  for bone tissue engineering applications. The nanocomposite scaffolds were synthesized by solvent casting/particulate leaching and freeze-drying approaches. Microscopic investigations showed generation of pores with an average size of 200-400μm after addition of ceramic phase. Energy dispersive X-ray analysis confirmed uniform distribution of ceramic phase in polycaprolactone matrix. FTIR results determined the binding type of zeolite nanoparticles to the polycaprolactone matrix as physical bonding. The results of mechanical tests showed the increase in young’s modulus after addition of ceramic phase (from 0.04 to 0.3 and 3 to 7 MPa, respectively). The hydrophilicity of polycaprolactone increased after adding nanozeolite and more weight loss was observed for scaffold containing 20% zeolite (53.52 6 1.6%) with an increase in the rate of hydroxyapatite formation. The results showed that the prepared scaffolds have potential for cancellous bone tissue engineering application.

کلیدواژه‌ها [English]

  • Zeolite
  • nanocomposite
  • Tissue engineering
  • Scaffold

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مواد پیشرفته در مهندسی
دوره 39، شماره 4
اسفند 1399
صفحه 77-94

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