مطالعه خواص زیستی شیشه زیست‌فعال 68S پایدار شده در دماهای °C 600-800 به‌منظور ارتقای زیست‌سازگاری آن در کاربردهای درمانی مهندسی‌بافت استخوان

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده مهندسی مواد، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران

چکیده

در این پژوهش شیشه زیست‌فعال 68S آلایش یافته با استرانسیم، در دماهای پایدارسازی 600 تا 800 درجه سانتی‌گراد به روش سل- ژل سنتز شد و تأثیر دمای پایدارسازی بر خواص زیست‌فعالی برون‎تنی آن، قبل و پس از 14 روز غوطه‌وری در محلول شبیه‌سازی شده بدن توسط آزمون‌های زیستی بررسی شد. طبق نتایج حاصل از آزمون‌های حرارتی، کاهش وزن نمونه تا دمای 620 درجه سانتی‌گراد به دلیل خروج نیترات از ساختار شیشه زیست‌فعال و ثبات کاهش وزن آن در دمای 700 درجه سانتی‌گراد مشاهده گردید. همچنین نتایج آزمون‌های طیف‌سنجی پراش پرتو ایکس، طیف‌سنجی تبدیل فوریه فروسرخ و تصاویر حاصل از میکروسکوپ الکترونی روبشی، حاکی از تشکیل لایه هیدروکسی‌آپاتیت در روز سوم و پنجم غوطه‌وری و افزایش ارتفاع آن‌ها تا روز 14 غوطه‌وری به‌ترتیب در نمونه 68S7C و 68S8C بود. ضمن این‌که با توجه به نتایج آزمون طیف‌سنجی پلاسمای جفت‌شده القایی، نرخ بالاتر تشکیل لایه هیدروکسی‌آپاتیت بر روی سطح نمونه 68S7C نسبت به نمونه 68S8C تأیید گردید. علاوه بر این طبق نتایج آزمون‌های سمیّت سلولی و فعالیت فسفات قلیایی، رشد و تکثیر سلولی سلول‌های استخوان‌ساز MC3T3-E1 در نمونه 68S7C نسبت به نمونه 68S8C به‌ترتیب 13 و 7 درصد بهبود یافت. بنابراین با توجه به نتایج آزمون‌های مذکور، نمونه 68S7C به دلیل پایدارسازی در دمای بهینه 700 درجه سانتی‌گراد، داشتن نرخ بالاتر تشکیل لایه هیدروکسی‌آپاتیت، بهبود رشد و تکثیر سلولی سلول‌های استخوان‌ساز MC3T3-E1 و به تبع آن ارتقا زیست‌سازگاری آن، به‌عنوان یک گزینه قابل اعتماد به‌منظور کاربردهای درمانی مهندسی‌بافت استخوان انتخاب و معرفی گردید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Study of the Biological Properties of 68S Bioactive Glass Stabilized at Temperatures of 600-800 °C to Improve its Biocompatibility in Bone Tissue Engineering Therapeutic Applications

نویسندگان [English]

  • A.H. Moghanian
  • R. Ashrafzadeh Shimi
  • M. Thaghafi Yazdi
  • N. Kolivand
Department of Materials Engineering, Imam Khomeini International University, Qazvin, Iran
چکیده [English]

In this research, 68S bioactive glass contaminated with strontium was synthesized at stabilization temperatures of 600-800 °C by sol-gel method, and the effect of stabilization temperature on the in vitro bioactive properties, before and after 14 days of immersion in Simulated body Fluid was investigated by characterization tests and bioassays. According to the results of thermal tests, the weight loss of the sample up to 620 °C was observed due to the removal of nitrate from the bioactive glass structure and the stability of its weight loss at 700 °C. The results of X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy indicated the formation of hydroxyapatite layer on the 3rd and 5th day of immersion and their height increase until the 14th day of immersion for 68S7C and 68S8C samples, respectively. Also, according to the results of the Inductively Coupled Plasma test, the higher formation rate of hydroxyapatite layer on the surface of the 68S7C sample compared to the 68S8C sample was confirmed. In addition, according to the results of cytotoxicity and alkaline phosphatase tests, the growth and cell proliferation of MC3T3-E1 osteogenic cells in the 68S7C sample compared to the 68S8C sample improved by 13% and 7%, respectively. Therefore, according to the results of the tests, the 68S7C sample due to its stabilization at the optimal temperature of 700 °C, the controlled rate of destruction of the glass network, having a higher formation rate of hydroxyapatite layer, improving the growth and cell proliferation of MC3T3-E1 bone-forming cells and consequently improving its biocompatibility, was selected and introduced as a reliable option for bone tissue engineering therapeutic applications.

کلیدواژه‌ها [English]

  • 68S bioactive glass
  • Strontium
  • Stabilization
  • Biocompatibility
  • Tissue engineering

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مواد پیشرفته در مهندسی
دوره 43، شماره 1
خرداد 1403
صفحه 67-80

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