ساخت و مشخصه‌یابی حسگر الکتروشیمیایی غیرآنزیمی مبتنی بر پلیمر قالب مولکولی به‌منظور پایش گلوکز در سیستم‌های پزشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران

چکیده

مقدمه و اهداف: دیابت یک بیماری مزمن است که در اثر اختلال در تنظیم سطح گلوکز خون ایجاد می‌شود. ازاین‌رو، پایش مستمر غلظت گلوکز، به‌ویژه در حین انجام فعالیت‌های روزانه، از اهمیت بالایی برخوردار است. در پژوهش حاضر، یک حسگر الکتروشیمیایی غیرآنزیمی مبتنی بر پلیمر قالب‌مولکولی برای شناسایی گلوکز خون طراحی و توسعه یافته است.
مواد و روش‌ها: در مرحله نخست، گرافن با استفاده از روش رسوب شیمیایی فاز بخار بر سطح نیکل سنتز شد. پس از انتقال گرافن به بستر پلی‌اتیلن‌ترفتالات و حذف نیکل با محلول کلرید آهن، فرایند پلیمریزاسیون الکتروشیمیایی دوپامین هیدروکلراید در حضور گلوکز بر سطح گرافن انجام شد. در ادامه، گلوکز با استفاده از محلول اسیدسولفوریک از ساختار حذف گردید و بدین ترتیب، ساختار پلیمر قالب‌مولکولی شکل گرفت.
یافته‌ها: نتایج طیف‌سنجی رامان نشان داد که گرافن سنتزشده، در گروه گرافن‌های کم‌لایه قرار دارد. همچنین، پس از تشکیل ساختار پلیمر قالب‌مولکولی و حذف گلوکز، مورفولوژی ساختار حفظ شد که بیانگر پایداری پلیمر پس از فرایند حذف می‌باشد. علاوه‌براین، خروج گلوکز از ساختار باعث افزایش هدایت الکتریکی شد که ناشی از ایجاد حفرات در سطح و تأییدی بر تشکیل موفق ساختار قالب‌مولکولی است. بر اساس نتایج حاصل از آزمون ولتامتری پالس تفاضلی، حسگر طراحی‌شده حد تشخیص 0/3 میکرومولار را در بازه خطی 0/2 تا 2 میکرومولار نشان داد.
نتیجه‌گیری: ادغام پلی‌دوپامین و گرافن در طراحی حسگر مبتنی بر پلیمر قالب‌مولکولی، موجب بهبود عملکرد آن در زمینه شناسایی الکتروشیمیایی گلوکز گردیده و نشان‌دهنده پتانسیل بالای این سامانه برای توسعه نسل جدیدی از حسگرهای غیرآنزیمی است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Fabrication and Characterization of a Non-Enzymatic Electrochemical Sensor Based on Molecularly Imprinted Polymer for Glucose Monitoring in Medical Systems

نویسندگان [English]

  • Zahra Ghasemi
  • Mahshid Kharaziha
  • Hamidreza Salimi
  • Keyvan Raeissi
  • Fathollah Karimzadeh
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

Introduction and Objectives: Diabetes is a chronic disease caused by disturbances in blood glucose levels. As a result, continuous and real-time glucose monitoring—especially during routine daily activities—remains essential for effective disease management. In the present study, a non-enzymatic electrochemical sensor incorporating a molecularly imprinted polymer (MIP) was developed for the selective and sensitive detection of glucose in blood samples.
Materials and Methods: Graphene was synthesized on a nickel substrate via chemical vapor deposition (CVD) and then transferred onto a polyethylene terephthalate (PET) film. The underlying nickel was subsequently etched away using an iron (III) chloride solution. To fabricate the MIP layer, dopamine hydrochloride was electropolymerized on the graphene surface in the presence of glucose as a template molecule. Following polymerization, the glucose was extracted with sulfuric acid, leaving behind molecularly imprinted cavities that serve as selective recognition sites for glucose detection.
Results: Raman spectroscopic analysis verified the few-layered structure of the synthesized graphene. The polymer morphology showed little change after template removal, suggesting that the structure remained stable. A notable reduction in the total resistance of the electrode following template removal was attributed to the generation of nanoscale cavities, confirming the successful formation of molecularly imprinted sites. Differential pulse voltammetry (DPV) analysis demonstrated a limit of detection of 0.3 μM and a well-defined linear response over the concentration range of 0.2 to 2 μM.
Conclusion: The combination of polydopamine and graphene in the MIP-based sensor notably improved its electrochemical response for glucose detection, highlighting its potential as a sensitive and enzyme-free sensing platform.

کلیدواژه‌ها [English]

  • Electrochemical sensor
  • Chemical vapor deposition
  • Molecularly imprinted polymer structure
  • Polydopamine
  • Glucose
  • Graphene

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مواد پیشرفته در مهندسی
دوره 45، شماره 1
(شماره پیاپی 52)
فروردین 1405
صفحه 1-15

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