مواد پیشرفته در مهندسی

مواد پیشرفته در مهندسی

پانسمان نانولیفی فیبروئین ابریشم-لیگنین جهت درمان زخم‌های دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان
امیرحسین محمدی فرد
مهشید خرازیها
مسعود عطاپور
دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران
10.47176/jame.45.3.1122
چکیده
مقدمه و اهداف: پوست به‌عنوان بیشترین عضوی که در معرض محیط بیرونی قرار دارد می‌تواند به راحتی به دلیل تروما، سوختگی، زخم، جراحی و بیماری‌های مزمن یا واکنش‌های پوستی التهابی مختل شود. هدف از انجام این پژوهش، ساخت و مشخصه‌یابی پانسمان نانوالیاف فیبروئین ابریشم-لیگنین جهت درمان زخم‌های دیابتی می‌باشد.
مواد و روش‌ها: در ابتدا فیبروئین ابریشم از پیله‌های کرم ابریشم استخراج شد و با نسبت‌های 1:5، 1:6 و 1:7 لیگنین، در ولتاژ 20 کیلوولت، نانوالیاف تولید گردید. سپس، نانوالیاف به مدت 30 دقیقه جهت انجام فرایند اتصال عرضی در اتانول 96 درصد غوطه‌ور شد. در گام نخست، به منظور بررسی ویژگی‌های مورفولوژی، از میکروسکوپ الکترونی روبشی استفاده گردید. سپس، جهت دستیابی به خواص یک پانسمان ایده‌آل، ارزیابی‌های مکانیکی، فیزیکی، آنتی‌اکسیدان، سلولی و ضدباکتریایی صورت گرفت.
یافته‌ها: نتایج نشان داد که الیاف کامپوزیتی در نسبت‌های مختلف یکنواخت بوده و دارای ابعاد نانومتری است. میزان تورم در نمونه‌های کامپوزیتی نسبت به نمونه فیبروئین ابریشم خالص از 37/53 ± 359/99 درصد به 63/47 ± 468/37 درصد رسید و پایداری مناسبی ارائه نمود. خواص آنتی‌اکسیدانی و ضدباکتریایی نشان داد، با اضافه شدن لیگنین، فعالیت آنتی‌اکسیدانی و ضدباکتری افزایش یافت. همچنین در بررسی زنده‌مانی سلولی پانسمان مورد نظر مشاهده شد که وجود لیگنین اثر نامطلوبی بر سلول‌ها نداشته و تکثیر و رشد سلول‌ روی سطح نمونه‌ها مشاهده شد.
نتیجه‌گیری: از آنجایی که جهت دستیابی به یک پانسمان ایده‌آل خواص زیستی حائز اهمیت است، این نانوالیاف به دلیل گروه‌های فنولی هیدروکسیل و متوکسی موجود در لیگنین، خواص منحصربه‌فردی را بر خواص فیزیکی، آنتی‌اکسیدان، ضد‌‌باکتریایی و چسبندگی سلولی ارائه می‌دهد که می‌تواند آن را گزینه مناسبی جهت کاربردهای زیست پزشکی از جمله بهبود و ترمیم زخم نماید.
کلیدواژه‌ها
پانسمان زخم دیابتی
لیگنین
فیبروئین ابریشم
الکتروریسی
ضدباکتری
آنتی‌اکسیدان
موضوعات

عنوان مقاله English

Silk Fibroin-Lignin Nanofiber Dressing for the Treatment of Diabetic Wounds

نویسندگان English

Amirhosein Mohammadifard
Mahshid Kharaziha
Masoud Atapour
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده English

Introduction and Objectives: As the largest organ exposed to the external environment, the skin is highly susceptible to disruption due to trauma, burns, wounds, surgical interventions, chronic diseases (e.g., diabetes), or inflammatory dermatological reactions. The aim of this study was to fabricate and characterize silk fibroin-lignin nanofiber dressings for the treatment of diabetic wounds.
Materials and Methods: Initially, silk fibroin was extracted from silkworm cocoons and nanofibers were fabricated at a voltage of 20 kV using lignin at ratios ranging from 1:5 and 1:6 and 1:7. Subsequently, the nanofibers were immersed in 96% ethanol for 30 minutes to carry out the crosslinking process. In the first step, scanning electron microscopy (SEM) was employed to investigate the morphological features. Then, mechanical, physical, antioxidant, cellular, and antibacterial evaluations were performed to assess the properties of an ideal wound dressing.
Results: The results revealed that the composite fibers were uniform in structure across different ratios and possessed nanometer-scale diameters. The swelling rate of the composite samples increased from 359.99 ± 37.53% in pure silk fibroin samples to 468.37 ± 63.47%, indicating favorable stability. Antioxidant and antibacterial assays demonstrated that the addition of lignin enhanced both antioxidant and antibacterial activities. Furthermore, cell viability assessments showed that the presence of lignin did not exert any detrimental effects on the cells, and cell proliferation and growth were observed on the surface of the samples.
Conclusion: Since achieving an ideal wound dressing requires critical biological properties, these nanofibers due to their hydroxyl and methoxy phenolic groups in lignin exhibit unique advantages in physical properties, antioxidant activity, antibacterial effects, and cell adhesion. These characteristics make them a promising candidate for biomedical applications, particularly wound healing, and tissue regeneration.

کلیدواژه‌ها English

Diabetic wound dressing
Lignin
Silk Fibroin
Electrospinning
Antibacterial
Antioxidant
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مواد پیشرفته در مهندسی
دوره 45، شماره 3
(شماره پیاپی 54)
پاییز 1405
صفحه 1-19

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