ساخت و مشخصه‌یابی پوشش کراتین بر زیرلایه منیزیمی جهت کاربرد کاشتنی استخوان

مقالات آماده انتشار

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران

چکیده

مقدمه و اهداف: پوشش‌های کراتین، به دلیل خواص منحصر‌به‌فرد خود، به عنوان یک راهکار نوین در کنترل نرخ تخریب و خوردگی زیرلایه و تقویت بازسازی استخوان مورد توجه قرار گرفته‌اند. هدف از پژوهش حاضر، توسعه پوشش کراتین روی آلیاژ منیزیم به منظور کنترل رفتار خوردگی آن است.
مواد و روش‌ها: در این پژوهش، کراتین از پر کبوتر براساس پروتکل ارائه‌شده در پژوهش‌های پیشین استخراج شد و جهت بهبود چسبندگی زیرلایه، فرایند قلیایی‌سازی آلیاژ پیش از پوشش‌دهی انجام شد. مورفولوژی و ضخامت پوشش کراتین با تغییر زمان الکترواسپری (۳۰، ، ۶۰ ، ۹۰ و  120 دقیقه) بهینه‌سازی شد. از طیف‌‌سنجی مادون قرمز، جهت تایید استخراج کراتین و به منظور بررسی مقاومت در برابر خوردگی نمونه‌ها، آزمون پلاریزاسیون پتانسیودینامیکی بر روی نمونه‌ها‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌ انجام شد. 
یافته‌ها: نتایج طیف‌سنجی مادون قرمز، پیوندهای پپتیدی را که با آمید یک (عدد موجی  cm-1 1635)، دو ( cm-1 1531) و سه (cm-1 1238 ) شناخته می‌شود را نشان می‌دهد و طبق نتایج، زاویه ترشوندگی در نمونه‌های حاوی پوشش کراتین بعد از (30، ، 60 ، 90 و 120دقیقه پوشش‌دهی) به 1± 54، 2 ± 49، 2 ± 45 و 0/5 ± 41 درجه کاهش پیدا می‌کند. 
نتیجه‌گیری: نتایج این پژوهش نشان‌دهنده اصلاح خواص سطحی، هم‌چون زبری و ترشوندگی و بهبود قابل‌توجه خواص خوردگی آلیاژ AZ91 با پوشش کراتین است. این بهبود به دلیل ایجاد یک مانع فیزیکی مؤثر و همچنین زیست‌سازگاری بالای کراتین است که می‌تواند کاربردهای گسترده‌ای در زمینه‌های پزشکی داشته باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Fabrication and Characterization of Keratin Coating on Magnesium Substrate for Bone Implant Application

نویسندگان [English]

  • Farnoosh Moeinipour
  • Mahshid Kharaziha
  • Masoud Atapour
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

Introduction and Objectives: Keratin coatings have attracted attention as a novel solution for controlling the rate of substrate degradation and corrosion and enhancing bone regeneration due to their unique properties. The aim of the present study is to develop a keratin coating on magnesium alloy in order to control its corrosion behavior.
Materials and Methods: In this study, keratin was extracted from pigeon feathers following the protocol described in previous research. To enhance substrate adhesion, the magnesium alloy was alkalized prior to coating. The morphology and thickness of the keratin coating were optimized by varying the electrospray duration (30, 60, 90, and 120 minutes). Fourier-transform infrared spectroscopy (FTIR) was employed to confirm the successful extraction of keratin, while potentiodynamic polarization tests were conducted to assess the corrosion resistance of the coated samples.
Results: The infrared spectroscopy results revealed characteristic peptide bonds corresponding to amide I (1635 cm⁻¹), amide II (1531 cm⁻¹), and amide III (1238 cm⁻¹). Furthermore, the water contact angle of samples coated with keratin for 30, 60, 90, and 120 minutes decreased progressively to 54 ± 1°, 49 ± 2°, 45 ± 2°, and 41 ± 0.5°, respectively, indicating enhanced surface wettability.
Conclusion: The results of this study demonstrate modifications in surface properties such as roughness and wettability, along with a significant enhancement in the corrosion resistance of AZ91 alloy coated with keratin. This improvement is attributed to the formation of an effective physical barrier and the excellent biocompatibility of keratin, suggesting its potential for broad applications in the medical field.

کلیدواژه‌ها [English]

  • AZ91 alloy
  • Keratin
  • Coating
  • Eelectrospray
  • Ccorrosion resistance

مراجع

  1. Zhu Y, Liu W, Ngai T. Polymer coatings on magnesium-based implants for orthopedic applications. J Polym Sci. 2022;60(1):32–51. https://doi.org/10.1002/pol.20210578
  2. Xue N, Ding X, Huang R, Jiang R, Huang H, Pan X, et al. Bone tissue engineering in the treatment of bone defects. Pharmaceuticals (Basel) 2022;15(7). https://doi.org/10.3390/ph15070879
  3. Safari N, Kharaziha M, Toroghinejad M, Kharaziha M, Saeedi V. Influence of Cu element on degradation rate and biological properties of Mg-Al-Cu alloy prepared by spark plasma sintering. J Adv Mater Eng. 2022;38(3):87–99. https://doi.org/47176/jame.38.3.20931
  4. Tong P, Sheng Y, Hou R, Iqbal M, Chen L, Li J. Recent progress on coatings of biomedical magnesium alloy. Smart Mater Med. 2022;3:104–16. https://doi.org/1016/j.smaim.2021.12.007
  5. Cuartas-Marulanda D, Forero Cardozo L, Restrepo-Osorio A, Fernández-Morales P. Natural coatings and surface modifications on magnesium alloys for biomedical applications. Polymers 2022;14. https://doi.org/10.3390/polym14235297
  6. Saha S, Lestari W, Dini C, Sarian MN, Hermawan H, Barão VAR, et al. Corrosion in Mg-alloy biomedical implants- the strategies to reduce the impact of the corrosion inflammatory reaction and microbial activity. J Magnes Alloy 2022;10(12):3306–26. https://doi.org/10.1016/j.jma.2022.10.025
  7. Li LY, Cui LY, Zeng RC, Li SQ, Chen XB, Zheng Y, et al. Advances in functionalized polymer coatings on biodegradable magnesium alloys – A review. Acta Biomater. 2018;79:23–36.

https://doi.org/10.1016/j.actbio.2018.08.030

  1. Nasr Azadani M, Zahedi A, Bowoto OK, Oladapo BI. A review of current challenges and prospects of magnesium and its alloy for bone implant applications. Prog Biomater. 2022;11(1). https://doi.org/1007/s40204-022-00182-x
  2. Perinelli DR, Cambriani A, Cespi M, Tombesi A. Exploring the functional properties of hydrolyzed keratin: Filling the knowledge gap on surface active, emulsifying, and thickening properties. ACS Omega 2025;10(11):10244-10257. https://doi.org/10.1021/acsomega.4c10755.
  3. Bhat HF, Amin N, Nasir Z, Nazir S, Bhat ZF, Malik AA, et al. Keratin as an effective coating material for in vitro stem cell culture, induced differentiation and wound healing assays. Heliyon 2024;10(5):e27197. https://doi.org/1016/j.heliyon.2024.e27197
  4. Ghafarzadeh M, Kharaziha M, Atapour M. Bilayer micro-arc oxidation-poly (glycerol sebacate) coating on AZ91 for improved corrosion resistance and biological activity. Prog Org Coat. 2021;161:106495. https://doi.org/10.1016/j.porgcoat.2021.106495
  5. Giuliani C, Pascucci M, Riccucci C, Messina E, Salzano de Luna M, Lavorgna M, et al. Chitosan-based coatings for corrosion protection of copper-based alloys: A promising more sustainable approach for cultural heritage applications. Prog Org Coat. 2018;122:138–46. https://doi.org/10.1016/j.porgcoat.2018.05.002
  6. Tavakoli Dehaghi S, Kharaziha M, Darvishi S, Nemati S, Kharaziha M. Preparation and characterization of PDMS-SiO2-CuO nanocomposite coating on stainless steel and its super-hydrophobicity property. J Adv Mater Eng. 2022;37(3):1–12. https://doi.org/29252/jame.37.3.1
  7. Soleimani F, Emadi R. Evaluation of bioactivity and corrosion behavior of AZ91 alloy with polymer/ceramic composite coating. J Adv Mater Eng. 2019;38(3):73–85.

https://doi.org/10.47176/jame.38.3.20201

  1. Ranjit E, Hamlet S, Love RM. Keratin coated titanium as an aid to osseointegration: Physicochemical and mechanical properties. Surf Coat Technol. 2023;462:129457. https://doi.org/10.1016/j.surfcoat.2023.129457
  2. Duncan WJ, Greer PFC, Lee MH, Loch C, Gay JHA. Wool-derived keratin hydrogel enhances implant osseointegration in cancellous J Biomed Mater Res B Appl Biomater. 2018;106(6):2447–54. https://doi.org/10.1002/jbm.b.34047
  3. Li J, Wu C, Wicks DA, Smith RA, Morgan SE. Preparation and characterization of keratin coatings for orthopedic implant titanium rods. In: Nalwa HS, editor. Cosmetic nanotechnology. ACS Symposium Series. Vol. 961. Washington (DC): American Chemical Society; 2007. p. 149-62. https://doi.org/10.1021/bk-2007-0961.ch008
  4. Ferraris S, Prato M, Vineis C, Varesano A, Gautier di Confiengo G, Spriano S. Coupling of keratin with titanium: A physico-chemical characterization of functionalized or coated surfaces. Surf Coat Technol. 2020;397:126057. https://doi.org/10.1016/j.surfcoat.2020.126057
  5. Spiridon I, Paduraru OM, Zaltariov MF, Darie RN. Influence of keratin on polylactic acid/chitosan composite properties. Behavior upon accelerated weathering. Ind Eng Chem Res. 2013;52(29):9822–33. https://doi.org/10.1021/ie400848t
  6. Chhipa SM, Sharma S, Kumar Bagha A. Recent development in polymer coating to prevent corrosion in metals: A review. Mater Today Proc. 2024. https://doi.org/1016/j.matpr.2024.09.001
  7. Toorani Farani M. Effect of plasma electrolytic oxidation process as a pretreatment on corrosion resistance of polymeric coatings applied on Mg alloy. J Adv Mater Eng. 2022;39(1):29–45. https:// doi.org/47176/jame.39.1.21581
  8. Rahman MK, Phung TH, Oh S, Kim SH, Ng TN, Kwon KS. High-efficiency electrospray deposition method for nonconductive substrates: Applications of superhydrophobic coatings. ACS Appl Mater Interfaces. 2021;13(15):18227-18236.

https://doi.org/10.1021/acsami.0c22867

  1. Nguyen T, Wortman P, He Z, Goulas J, Yan H, Mokhtari M, et al. Achieving superhydrophobic surfaces via air-assisted electrospray. Langmuir 2022;38(9):2852-2861. https://doi.org/10.1021/acs.langmuir.1c03134
  2. Tang H, Wu T, Xu F, Tao W, Jian X. Fabrication and characterization of Mg(OH)2 films on AZ31 magnesium alloy by alkali treatment. Int J Electrochem Sci. 2017;12(2):1377–88. https://doi.org/10.20964/2017.02.35
  3. Arungandhi K, Sunmathi D, Jeyaraj N, Nanthavanan P. Biological synthesis of keratin nanoparticles from dove feather (Columba livia) and its applications. Asian J Pharm Clin Res. 2019;12(10):350 https://doi.org/10.22159/ajpcr.2019.v12i10.34572
  4. Pourjavaheri F, Ostovar Pour S, Jones OAH, Smooker PM, Brkljača R, Sherkat F, et al. Extraction of keratin from waste chicken feathers using sodium sulfide and l-cysteine. Process Biochem. 2019;82:205–14. https://doi.org/10.1016/j.procbio.2019.04.010
  5. Cheng S, Wang W, Li Y, Gao G, Zhang K, Zhou J, et al. Cross-linking and film-forming properties of transglutaminase-modified collagen fibers tailored by denaturation temperature. Food Chem. 2019; 271:527–35. https://doi.org/10.1016/j.foodchem.2018.07.223
  6. Schwartz Z, Nasazky E, Boyan BD. Surface microtopography regulates osteointegration: the role of implant surface microtopography in osteointegration. Alpha Omegan 2005;98(2):9–19.
  7. Alaei M, Atapour M, Labbaf S. Electrophoretic deposition of chitosan-bioactive glass nanocomposite coatings on AZ91 Mg alloy for biomedical applications. Prog Org Coat. 2020;147:105803. https://doi.org/10.1016/j.porgcoat.2020.105803
  8. Kim MJ, Choi MU, Kim CW. Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell. Biomaterials 2006;27(32):5502–11. https://doi.org/1016/j.biomaterials.2006.06.023
  9. Wu F, Liang J, Li W. Electrochemical deposition of Mg(OH)2/GO composite films for corrosion protection of magnesium alloys. J Magnes Alloy. 2015;3(3):231–6. https://doi.org/1016/j.jma.2015.08.004
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مقالات آماده انتشار، پذیرفته شده
انتشار آنلاین از تاریخ 09 مهر 1404

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