ساخت و مشخصه‌یابی حسگر الکتروشیمیایی سلول‌های CD8+Tمبتنی بر کامپوزیت پلی‌پیرول و اکسید گرافن کاهش‌یافته

مقالات آماده انتشار

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده مواد. دانشگاه صنعتی اصفهان. اصفهان. ایران

چکیده

مقدمه و اهداف: شناسایی نشانگر زیستی سلول‌های CD8+T، یک شاخص کلیدی بیماری‌های ایمونولوژی، با روش‌های فعلی زمان‌بر است. هدف از این پژوهش، توسعه یک حسگر زیستی الکتروشیمیایی حساس، برپایه نانوکامپوزیت پلی‌پیرول و اکسید گرافن کاهش‌یافته جهت تشخیص سلول‌های CD8+T با قابلیت مراقبت در محل، دقت، حساسیت بسیار مناسب و ساده می باشد.
مواد و روش‌ها: فرایند رسوب‌دهی کامپوزیت پلی‌پیرول و اکسید گرافن کاهش‌یافته با تکنیک ولتامتری چرخه‌ای، با تعداد سیکل‌های 15، 20 و 25 (کد نمونه‌های A15، A20 و A25) روی الکترود کار حسگر انجام شد. برای تهیه نمونه با کامپوزیت پلی‌پیرول و اکسید گرافن با تعداد سیکل بهینه تحت لامپ فرابنفش (کد نمونه B20)، تکرار و نمونه‌ها مشخصه‌یابی شدند. آنتی‌بادی روی الکترود توسعه یافته ایموبلایز گردید و عملکرد حسگر با آزمون ولتامتری پالسی تفاضلی انجام شد.
یافته‌ها: تصاویر میکروسکوپی الکترونی روبشی گسیل میدانی نشان داد که نمونه A20 در مقایسه با نمونه B20، دارای مورفولوژی سطحی یکنواخت‌تر و متخلخل‌تر است. براساس نتایج ولتامتری پالس تفاضلی، افزایش غلظت سلول‌های CD8+T، منجر به کاهش شدت جریان شد. با توجه به منحنی کالیبراسیون، حد تشخیص برابر با هفت سلول در میلی‌لیتر تعیین شد که نشان‌دهنده حساسیت بالای حسگر در شناسایی سلول‌های CD8+T است.
نتیجه‌گیری: نمونه A20، به عنوان ساختار بهینه برای کاربرد در حسگرهای زیستی الکتروشیمیایی شناسایی شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Fabrication and Characterization of an Electrochemical CD8⁺ T Cells Sensor Based on Polypyrrole/Reduced Graphene Oxide Composite

نویسندگان [English]

  • maryam Amoo
  • hamidreza salimi jazi
  • Sheyda Labbaf
  • Fathallah Karimzadeh
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

Introduction and Objectives: Detecting the CD8⁺ T cell biomarker—a key indicator of immunological diseases—is often slow with current methods. This research aims to develop a sensitive, accurate, and easy-to-use electrochemical biosensor, utilizing reduced graphene oxide and polypyrrole nanocomposite, for on-site, sensitive, accurate, and rapid detection of CD8⁺ T cells.
Materials and Methods: The polypyrrole/reduced graphene oxide composite was deposited onto the sensor's working electrode using cyclic voltammetry with 15, 20, and 25 cycles, corresponding to sample codes A15, A20, and A25. To prepare the sample containing the polypyrrole and graphene oxide composite with the selected number of cycles under a UV lamp (sample code B20), the same procedure was repeated, followed by characterization of the samples. Antibody immobilization was then performed on the developed electrode, and sensor performance was assessed using differential pulse voltammetry.
Results: FESEM imaging revealed that the sample A20 had a more uniform and porous surface morphology compared to the sample B20. According to the differential pulse voltammetry results, an increase in the concentration of CD8⁺ T cells led to a decrease in current intensity. Based on the calibration curve, the detection limit was determined to be seven cells.ml-1, indicating the high sensitivity of the sensor in identifying CD8⁺ T cells.
Conclusion: Sample A20 was identified as the optimal structure for application in electrochemical biosensors based on Ppy-rGo composite to detect CD8+T cells.

کلیدواژه‌ها [English]

  • Polypyrrole
  • Reduced graphene oxide
  • Electrochemical sensor
  • CD8⁺ T Cells
  • Cyclic voltammetry
  • Differential pulse voltammetry

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انتشار آنلاین از تاریخ 30 مهر 1404

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