ساخت و مشخصه‌یابی نانو کامپوزیت پلی‌کاپرولاکتان/ هیدروکسید ‌دوگانه لایه‌ای به‌منظور کاربرد در مهندسی بافت سخت

نویسندگان

1 1- پژوهشکده زیست فناوری پزشکی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران

2 2- گروه بیومواد، دانشکده مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران

3 3- دانشکده علوم، دانشگاه بین‌‌المللی امام خمینی (ره)، قزوین

چکیده

در سال‌های اخیر استفاده از نانومواد در داربست‌های مهندسی بافت استخوان به‌دلیل تقلید از ساختار بافت طبیعی استخوان که دارای یک ساختار نانوکامپوزیتی درهم آمیخته با یک ماتریس سه بعدی است، مورد توجه قرار گرفته است. در این میان، پلی­کاپرولاکتان به‌عنوان یک زیست پلیمر، درساخت داربست‌های مهندسی بافت استخوان مورد استفاده قرار گرفته است. هدف از این پژوهش، ساخت داربست نانوکامپوزیتی پلی­کاپرولاکتان/ هیدروکسید دوگانه لایه­ای با خواص مکانیکی، زیست فعالی و زیستی مناسب برای کاربرد در مهندسی بافت استخوان اسفنجی است. برای ساخت داربست­ها از ترکیب دو روش فروشویی ذره­ای و خشکایش انجمادی و هم‌چنین برای مطالعات سلولی از سلولMG63 (استئوسارکومای استخوان) استفاده شد. تحلیل طیف سنج طول موج انتشاری از نمونه‌ها، توزیع یکنواخت فاز سرامیکی در بستر پلی کاپرولاکتان را تأیید کرد. نتایج بررسی مکانیکی داربست­ها حاکی از افزایش مدول یانگ بعد از اضافه شدن فاز سرامیکی بود. بررسی‌های میکروسکوپی نشان داد که داربست­ها از تجمع ریزکره­ها پس از اضافه شدن فاز سرامیکی حاصل شدند و اندازه تخلخل‌ها بین 100 تا 600 میکرومتر گزارش شد. هم‌چنین با افزودن فاز سرامیکی آب‌دوستی پلی کاپرولاکتان افزایش یافت، اما تشکیل هیدروکسی آپاتیت در محیط شبیه‌سازی شده بدن، به‌علت وجود یون منیزیم به‌تأخیر افتاد. ارزیابی‌های سلولی، اتصال سلول­ها و تکثیرشان روی داربست­ها را تأیید کردند. نتایج نشان می­دهد که داربست‌های ساخته شده قابلیت کاربرد در مهندسی بافت استخوان اسفنجی را دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Preparation and Characterization of Polycaprolactone / Layered Double Hydroxide Nanocomposite for Hard Tissue Engineering Applications

نویسندگان [English]

  • M. Baradaran 1
  • S.S. Shafiei 2
  • F. Moztarzadeh 2
  • S.Z. Mortazavi 3
1 1- Stem cell and Regenerative Medicine Department, Institute of Medical biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
2 2- Biomaterial Group, Biomedical Engineering Faculty, Amirkabir University of Technology, Tehran, Iran
3 3- Physics Group, Faculty of Science, Imam Khomeini International University, Qazvin, Iran
چکیده [English]

In recent years the use of nanomaterials in bone tissue engineering scaffold has been considered due to its imitating the structure of natural bone tissue which contains a nanocomposite structure mixed with a three-dimensional matrix. In the meantime, Polycaprol actone has been used as a bio-polymer in bone tissue engineering applications as a scaffold. The aim of this study is to develop porous scaffolds made of polycaprol actone/layered double hydroxide biocomposite, with appropriate mechanical, bioactive and biological properties, for bone tissue engineering application. The nanocomposite scaffolds were fabricated by the particulate leaching method and freeze-drying method. In this study, MG63 cells (osteosarcoma) was investigated for cellular study. Energy dispersive X-ray analysis confirmed uniform distribution of ceramic phase in polycaprol actone matrix. The results of mechanical tests showed the increase in young’s modulus after addition of ceramic phase. The microscopic investigations demonstrated that the pores generated after addition of ceramic phase and the average size of pores was as large as 100-600μm. Also by the addition of LDH, the hydrophilicity of PCL increased but the rate of hydroxyapatite formation was delayed due to presence of magnesium ions. The cell culture experiments confirmed the attachment and proliferation of cells on the scaffolds. The results showed that the fabricated scaffolds have the potential to be used in cancellous bone tissue engineering.

کلیدواژه‌ها [English]

  • Layered double hydroxide
  • composite
  • Freeze-drying
  • Tissue engineering
  • Scaffold

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مواد پیشرفته در مهندسی
دوره 35، شماره 3
آذر 1395
صفحه 13-28

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