بررسی تأثیر نانوذرات هیدروکسی آپاتیت بر خواص نانوالیاف کراتین جهت کاربرد در مهندسی بافت

نویسندگان

1 1. گروه مهندسی پزشکی، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران

2 2. گروه شیمی، واحد یزد، دانشگاه آزاد اسلامی، یزد، ایران

چکیده

در این تحقیق، داربست کراتین/ پلی کاپرولاکتون/ هیدروکسی آپاتیت HA)) با روش الکتروریسی ساخته شد. سپس تأثیر نانوذرات  HAبر خواص داربست B (کراتین33 درصد، پلی کاپرولاکتون50 درصد و هیدروکسی آپاتیت17 درصد) و داربست A (کراتین 40 درصد و پلی‌کاپرولاکتون 60 درصد) مورد بررسی قرار گرفت. مورفولوژی سطح، گروه‌های عاملی موجود بر سطح نمونه، درصد تخلخل و سطح ویژه داربست‌ها به‌ترتیب با میکروسکوپی الکترونی روبشی، طیف‌سنجی مادون قرمز تبدیل فوریه، روش جابه‌جایی مایع و آزمون BET ارزیابی ‌شد. متوسط قطر الیاف در نمونه   Aو B به‌ترتیب 184 و 108 نانومتر محاسبه شد. همچنین، نتایج آزمون‌ها حاکی از افزایش سطح ویژه داربست حاوی نانوذرات HA نسبت به داربست بدون نانوذرات HA تا تقریباً به میزان دو برابر بودند. با بررسی رفتار زیست تخریب‌پذیری داربست‌ها در محلول بافر فسفات، افزایش میزان کاهش وزن در داربست B مشاهده ‌شد. درصد زنده‌مانی و چسبندگی سلول‌های استخوانی رده سلولی 2‌Saos- بر سطح داربست ها با روش MTT بررسی شد و افزایش رشد سلول ها بر سطح داربست PCl/Kr حاوی نانوذرات هیدروکسی آپاتیت مشاهده شد. بنابراین، داربست حاوی نانوذرات هیدروکسی آپاتیت می‌تواند گزینه مناسبی برای کاربرد درمهندسی بافت باشد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Hydroxyapatite Nanoparticles on Properties of Keratin/Poly Caprolactone Nanofibers for Tissue Engineering

نویسندگان [English]

  • M. Mirhaj 1
  • M. Mahmoodi 1
  • A. Shybani 2
1 1. Department of Biomedical Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.
2 2. Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Iran.
چکیده [English]

In this research, keratin (Kr)/ poly caprolactone (PCL)/ hydroxyapatite (HA) scaffold was made by electrospinning method. Then, the effect of HA nanoparticles on properties of scaffold B (Kr 33%, PCL 50% and HA 17%) and scaffold A (Kr 40% and PCL 60%) were studied. The surface morphology, functional groups on the surface of samples, porosity, and specific surface area were evaluated by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrophotometer (FTIR), liquid displacement method, and BET test, respectively. The mean diameter of fibers in samples A and B was measured 184 nm and 108 nm, respectively. Results showed that the  specific surface area in scaffolds with HA nanoparticles was almost 2 times higher than that of the scaffold without HA. The biodegradability of scaffolds was examined in phosphate buffer solution (PBS) and the results showed an increase in the weight loss percentage of the scaffold B. The cell viability and adherence of osteosarcoma cell line (Saos-2) on the scaffold surface was observed via MTT assay and the results showed an increase in cell growth on PCL/Kr scaffolds with HA nanoparticles. Thus, scaffolds containing HA nanoparticles can be a good choice for tissue engineering applications.

کلیدواژه‌ها [English]

  • Keratin
  • hydroxyapatite
  • electrospinning
  • Nanofibers
  • Tissue engineering

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مواد پیشرفته در مهندسی
دوره 36، شماره 4
اسفند 1396
صفحه 45-57

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