ساخت داربست‌های لایه‎‌به‌لایه نانو‌لیفی تهیه شده از پلی‌کاپرولاکتون و پلیمرهای آب‌دوست و بتا تری‌کلسیم فسفات برای مهندسی بافت

نویسندگان

1 1. دانشکده فنی، گروه مهندسی نساجی، دانشگاه گیلان

2 2. گروه آناتومی، دانشکده پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران

چکیده

در این پژوهش با استفاده از پلیمرهای زیست‌تخریب‌پذیر، داربست‌های نانو‌لیفی از الکتروریسی دو نازل شامل پلی‌کاپرولاکتون، پلی‌وینیل ‌پیرولیدون و پلی‌کاپرولاکتون، پلی‌وینیل­الکل و بتا تری‌کلسیم فسفات به‌طور متناوب و لایه‌به‌لایه تولید شد. بعد از تهیه داربست، از آزمون‌های میکروسکوپ الکترونی روبشی ((SEM، تورم، تخلخل، خواص مکانیکی و ارزیابی رفتار زیست‌تخریب‌پذیری در محلول نمک فسفات با خاصیت بافری، استفاده شد که نتایج آزمون‌ها زیست‌فعالی و خواص مکانیکی مناسب داربست لایه‌به‌لایه را تأیید می‌کند. مقادیر جذب آب با افزودن پلیمرهای آب‌دوست افزایش پیدا می‌کند و در داربست لایه‌به‌لایه به 214±811 درصد می‌رسد که اختلاف معناداری نسبت به پلی‌کاپرولاکتون خالص دارد. آزمون سنجش سمیت سلولی (MTT) روی داربست لایه‌به‌لایه بعد از گذشت 3، 5 و7 روز کشت سلول‌های بنیادی مغز استخوان موش صحرایی (rMSC) درصد بقای سلولی بالای 80 درصد را نشان می‌دهد و ریخت‌شناسی سلول‌ها روی داربست نشان‌دهنده قابلیت زیست‌سازگاری مطلوب سلول­ها روی داربست است.

کلیدواژه‌ها

عنوان مقاله [English]

Fabrication of Poly(ε-Caprolactone), Hydrophilic and β-Tricalcium Phosphate Layer- by -Layer Nanofibrous Scaffolds for Tissue Engineering

نویسندگان [English]

  • M. Sohrabi 1
  • M. Abbasi 1
  • M. M Ansar 2
1 1. Faculty of Engineering, Department of Textile Engineering, University of Guilan, Gilan, Iran.
2 2. Department of Anatomical Sciences, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
چکیده [English]

In this study, using biodegradable polymers, nanofiberouse scaffolds were fabricated from the layer-by-layer electrospinning method, including two layer that poly (ε-caprolactone), polyvinylpyrrolidone deposited at first layer and poly (ε-caprolactone), polyvinyl alcohol , β-tricalcium phosphate at latter. After prepration of scaffolds, scanning electron microscopy (SEM), swelling, porosity, mechanical properties and biodegradability behavior in buffered saline phosphate solution were  studied. The results confirmed the bioactivity  and suitable mechanical properties of the layer-by-layer scaffold. The swelling increase with the addition of hydrophilic polymers and reache 811 ± 214 % in the layer-by-layer scaffold, which reveald a significant difference compared to pure PCL. The MTT test on the layer-by-layer scaffold, after 3, 5 and 7 days of rats bone marrow stem cells (rMSC) culture, showed the cell viability of above 80% moreover, cells morphology on the scafold indicated the optimal compatibility of  cells on the scaffold.

کلیدواژه‌ها [English]

  • Scaffold
  • Layer- by -layer electrospinning
  • Poly(ε-caprolactone)
  • β-Tricalcium phosphate
  • Tissue engineering

مراجع

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مواد پیشرفته در مهندسی
دوره 38، شماره 1
خرداد 1398
صفحه 23-35

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