پانسمان نوین الیافی پلی‌کاپرولاکتون/ژلاتین حاوی ویتامین C و داروی آنتی‌باکتریال جهت درمان زخم‌های پوستی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی بافت، دانشگاه آزاد اسلامی واحد نجف آباد

2 2- گروه بیومتریال، نانو تکنولوژی و مهندسی بافت، دانشکده فناوری‌های نوین علوم پزشکی، دانشگاه علوم پزشکی اصفهان 3- مرکز تحقیقات بیوسنسور، دانشگاه علوم پزشکی اصفهان

3 گروه بیوشیمی، دانشگاه آزاد اسلامی واحد نجف آباد

چکیده

زخم‌های پوستی ناشی از سوختگی، آسیب‌های فیزیکی و بیماری‌هایی مانند دیابت در اثر عفونت و عدم رگ‌زایی مناسب می‌توانند منجر به آسیب‌های قطع عضو و حتی مرگ و میر افراد شوند. در این تحقیق، ترکیب پلیمر‌های پلی‌کاپرولاکتون و ژلاتین جهت الکتروریسی تهیه شده و داروی سیپروفلوکساسین و ویتامین C به‌صورت مجزا بارگذاری شد. ابتدا جهت بررسی ویژگی‌های مورفولوژی از میکروسکوپ الکترونی روبشی استفاده شد و سپس ارزیابی زاویه تماس، میزان تورم، نرخ تخریب و استحکام کششی صورت گرفت. همچنین، پاسخ سلول‌های فیبروبلاست از نظر تکثیر و چسبندگی سلولی بررسی شد. نتایج نشان داد که الیاف زخم پوش فاقد و حاوی دارو یکنواخت بوده و به‌ترتیب دارای قطری برابر با 244±1039/96 و 406±1403/72 نانومتر است. همچنین پانسمان حاوی و فاقد داروها محدوده وسیعی از قابلیت تورم و ماندگاری در بازه زمانی 35 روزه را نشان داد. خواص مکانیکی نمونه‌ها نیز نشان داد، با اضافه شدن داروی سیپروفلوکساسین و ویتامین C و تغییرات موفولوژی ناشی از آن، می‌توان استحکام کششی را تا 3/5 برابر افزایش داد. در بررسی سلول سازگاری زخم پوش مورد نظر مشاهده شد که وجود دارو در سامانه هیچ‌گونه اثر نامطلوبی بر سلول‌ها نداشته و تکثیر، رشد و چسبندگی سلول‌ فیبروبلاست پوستی بر روی سطح نمونه مشاهده ‌شد. ضمناً ترکیب پلیمر‌های ژلاتین و پلی‌کاپرولاکتون بدون نیاز به اتصال عرضی می‌تواند روند تخریب مناسب و رهایش مناسب برای دارو‌های بارگذاری شده را مهیا کند در‌حالی که خواص مکانیکی و انعطاف‌پذیری نیز در حد مطلوب حفظ شود. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

A Novel Fibrous Polycaprolactone/Gelatin Wound Dressing Containing Vitamin C and Antibacterial Drug For Wound Healing

نویسندگان [English]

  • Z. Ashuri 1
  • M. Tayibi 1
  • A. Zargar Kharazi 2
  • E. Pourazizi 3
1 Department of Tissue Engineering, Islamic Azad University, Najaf Abad branch,Najafabad, Iran
2 2- Department of Biomaterials, Nanotechnology and Tissue Engineering, Faculty of Modern Medical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran 3- Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Biochemistry, Islamic Azad University, Najaf Abad branch, Najafabad, Iran
چکیده [English]

Skin wounds caused by burns, physical injuries, and diseases such as diabetes can lead to amputation and even death of people due to infection and lack of proper angiogenesis. In this research, a wound dressing was made using the electrospinning method from a blend of poly-caprolactone and gelatin polymers which Ciprofloxacin and Vitamin C were loaded in the polymers separately. The morphology of the structure was observed by scanning electron microscope followed by evaluation of hydrophilicity, swelling, degradation rate, tensile strength, and drug release pattern. Fibroblast cell response was also considered using cytotoxicity test method. Results showed that the wound dressing fibers without drugs and containing drugs were uniform with a diameter of 1039.246 ± 96 and 1403± 406 nm, respectively. The wound dressing showed a suitable wide range of swelling ability and durability within 35 days. The tensile strength increased up to 3.5times by addition of ciprofloxacin and vitamin C due to the morphological changes. The presence of drugs in the system did not indicate any adverse effect on the cell's viability, and suitable proliferation and  adhesion of skin fibroblast cells were observed on the surface of the sample.  In addition,  a combination  of gelatin and polycaprolactone polymers could provide an appropriate degradation rate and release pattern without any cross-linking process, while preserving the mechanical properties and flexibility.

کلیدواژه‌ها [English]

  • Gelatin
  • Poly-caprolactone
  • Wound dressing
  • Drug release
  • Ascorbic acid
  • Ciprofloxacin

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مواد پیشرفته در مهندسی
دوره 43، شماره 1
خرداد 1403
صفحه 13-26

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