ساخت و مشخصه‌یابی هیدروژل کامپوزیتی فیبروئین ابریشم اصلاح شده/ چارچوب فلزی– آلی مبتنی بر روی با استفاده از واکنش فنتون برای کاربردهای پزشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده مهندسی مواد، دانشگاه صنعتی اصفهان، اصفهان، ایران

چکیده

مقدمه و اهداف: هیدروژل‌ها به‌عنوان داربست‌های سه‌بعدی آب‌دوست، نقش مهمی در جذب مایعات زیستی و ایجاد پایداری مکانیکی در فرآیند بازسازی بافت ایفا می‌کنند. هدف این پژوهش، طراحی و سنتز هیدروژل‌های کامپوزیتی جدیدی بر پایه فیبروئین ابریشم اصلاح‌شده با گروه‌های تیول و چارچوب فلزی-آلی (Zn-Bio MOF) حاوی یون روی و لیگاند آدنین، به‌منظور بهبود خواص مکانیکی و زیستی برای کاربردهای مهندسی بافت است.
مواد و روش‌ها: فیبروئین ابریشم خالص استخراج و با افزودن گروه‌های تیول از طریق اتصال ال-سیستئین اصلاح شد. برای تأیید این اصلاحات، طیف‌سنجی مادون‌قرمز تبدیل فوریه انجام شد.Zn-Bio MOF  نیز از طریق فرآیند هیدروترمال با یون روی (II) و لیگاند آدنین سنتز شد. سه نوع هیدروژل کامپوزیتی تهیه شد: (1) هیدروژل بر پایه فیبروئین ابریشم اصلاح‌شده، (2) هیدروژل بر پایه فیبروئین ابریشم اصلاح‌شده ژل‌شده با واکنش فنتون و (3) هیدروژل کامپوزیتی حاوی Zn-Bio MOF در غلظت‌های مختلف. برای مشخصه‌یابی، از میکروسکوپ الکترونی روبشی و پراش پرتو ایکس استفاده شد.
یافته‌ها: نتایج طیف‌سنجی مادون‌قرمز تبدیل فوریه کاهش شدت پیک‌های cm⁻¹ 1650 و cm⁻¹ 3300 و ظهور پیک‌های جدید مربوط به پیوندهای S-S و S-H را تأیید کرد. میانگین قطر نانوذرات Zn-Bio MOF برابر با 0/12±0/39 نانومتر و مساحت سطح ویژه آن 1138/3 مترمربع بر گرم بود. بررسی خواص تورم و تخریب نشان داد که هیدروژل‌های حاوی نانوذرات Zn-Bio MOF دارای مدول فشاری و چقرمگی بالاتر، کاهش وزن حدود 30 درصد طی 72 ساعت، و رفتار تورمی کنترل‌شده بودند.
نتیجه‌گیری: نتایج این پژوهش نشان می‌دهد که هیدروژل‌های کامپوزیتی Zn-Bio MOF به دلیل بهبود خواص مکانیکی و کنترل تورم، پتانسیل بالایی برای استفاده در مهندسی بافت و کاربردهای زیستی دارند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Fabrication and Characterization of Modified Silk Fibroin/Zinc-Based Metal-Organic Framework Composite Hydrogel Using Fenton Reaction for Medical Applications

نویسندگان [English]

  • Mohammad Kian Vojdanpak
  • Kiana Mohagheghian
  • Mahshid Kharaziha
Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده [English]

Introduction and Objectives: Hydrogels, as three-dimensional hydrophilic scaffolds, play a crucial role in absorbing biological fluids and providing mechanical stability in the tissue regeneration process. This study aims to design and synthesize novel composite hydrogels based on thiol-modified silk fibroin and a zinc-containing bio-metal-organic framework (Zn-Bio MOF) with an adenine ligand to enhance mechanical and biological properties for tissue engineering applications.
Materials and Methods: Pure silk fibroin was extracted and modified with thiol groups via L-cysteine conjugation. Fourier-transform infrared (FTIR) spectroscopy was conducted to confirm these modifications. The Zn-Bio MOF was synthesized through a hydrothermal process using zinc (II) ions and an adenine ligand. Three types of composite hydrogels were prepared: (1) hydrogel based on thiol-modified silk fibroin, (2) hydrogel based on thiol-modified silk fibroin crosslinked via the Fenton reaction, and (3) composite hydrogel containing Zn-Bio MOF at various concentrations. Material characterization was performed using scanning electron microscopy (SEM) and X-ray diffraction (XRD).
Results: FTIR spectroscopy confirmed the reduction in peak intensities at 1650 cm⁻¹ and 3300 cm⁻¹ and the appearance of new peaks corresponding to S-S and S-H bonds. The average particle size of Zn-Bio MOF was 0.39 ± 0.12 nm, with a specific surface area of 1138.3 m²/g. Swelling and degradation analyses indicated that Zn-Bio MOF-containing hydrogels exhibited improved mechanical properties, including higher compressive modulus and toughness, approximately 30% weight loss over 72 hours, and controlled swelling behavior.
Conclusion: The findings of this study suggest that Zn-Bio MOF composite hydrogels, due to their enhanced mechanical properties and controlled swelling behavior, hold significant potential for tissue engineering and biomedical applications.

کلیدواژه‌ها [English]

  • Silk
  • Metal-organic framework
  • Zinc
  • Fenton reaction
  • Hydrogel

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