بهینه‌سازی پارامتر‌های فرایند ساخت داربست کامپوزیت شیشه‌ زیست‌فعال آنتی‌باکتریال/پلی‌کاپرولاکتون به روش چاپ سه‌بعدی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی مواد، دانشکده مهندسی معدن و متالورژی، دانشگاه یزد، یزد، ایران

2 گروه بیومواد، نانوتکنولوژی و مهندسی‌بافت، دانشکده فناوری‌های نوین علوم پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

 در این مطالعه، داربست‌ سه‌بعدی کامپوزیتی شیشه زیست‌فعال حاوی دو درصد مولی نقره/ پلی‌کاپرولاکتون به‌کمک پرینتر سه بعدی با مزایای تکرار‌پذیری و انعطاف‌پذیری بالا در شکل و اندازه ساخته شد. پارامترهای تأثیرگذار (پارامتر‌های چاپگر و نسبت فاز شیشه، فاز پلیمر و حلال در جوهر پرینتر) برای چاپ داربست نانوکامپوزیتی با روش تاگوچی تعیین شدند. مشخصه‌یابی داربست‌های چاپ شده با کیفیت بهینه به‌کمک تفرق اشعه ایکس، میکروسکوپ الکترونی جاروبی، طیف‌سنجی مادون قرمز، تست زیست فعالی، طیف‌سنجی نشر اتمی، تست سمیّت و تکثیر سلولی انجام گرفت. نتایج مربوط به سنتز شیشه زیستی حاوی نقره به روش سل-ژل و عملیات حرارتی شده در دمای 550 درجه سانتی‌گراد، نانو ذراتی با قطر متوسط کمتر از 15 نانومتر و توزیع همگن نقره در آن را می‌دهد. شرایط بهینه برای چاپ داربست با کیفیت قابل قبول (درصد، اندازه و نحوه توزیع حفره‌ها، ساختار منظم لایه‌ها بر روی یکدیگر و تکرار‌پذیری)، نسبت فاز پلیمر به پودر شیشه معادل یک به دو، غلظت 50 درصد پلیمر در حلال، ریترکشن 1/5 و درایو جییر 1200 است. داربست ساخته شده در شرایط بهینه خواص ضد باکتریایی قابل توجه، خاصیت زیست‌فعالی خوب، قابلیت زنده ماندن سلولی قابل قبولی و فعالیت آلکالین فسفاتاز بالایی از خود را نشان دادند. بدین ترتیب داربست‌های نانوکامپوزیتی چاپ سه‌بعدی با حفره‌های ماکرو (تا 500 میکرون) و میکرو سایز و درصد تخلخل تا 64 درصد در ساختار می‌توانند کاندیدای امیدوارکننده‌ای برای مهندسی بافت استخوان باشند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Optimization of the Process Parameters of Antibacterial Bioactive Glass/Polycaprolactone Composite Scaffold Printed by 3D Method

نویسندگان [English]

  • Z. Golnia 1
  • M. Kalantar 1
  • M. Rafienia 2
  • A. Poursamar 2
1 Department of Mining and Metallurgical Engineering, Yazd University, Yazd, Iran
2 Faculty of New Technologies of Medical Sciences, Isfahan University, Isfahan, Iran
چکیده [English]

In this study, a 3D bioactive glass composite scaffold containing 2 mol% silver/polycaprolactone (PCL) was synthesized by a 3D printer with the advantages of reproducibility and high flexibility in shape and size. The effective parameters (printer parameters, ratio of glass-phase, polymer phase, and solvent in printer ink) were determined for printing of nanocomposite scaffold by Taguchi method. Characterization of printed scaffolds was performed using X-ray diffraction, scanning electron microscope, infrared spectroscopy, bioactivity test, atomic emission spectroscopy, toxicity test, and cell proliferation. The results related to the synthesis of silver-containing bioglass by sol-gel method and heat treated at 550°C offered nanoparticles with an average diameter of less than 15 nm and a homogeneous distribution of silver in the matrix. Ratio of polymer phase to glass powder equivalent to 0.5, concentration of polymer in solvent of 50%, retraction of 1.5, and drive gear of 1200 are obtained as the optimum conditions for scaffold printing with acceptable quality (percentage, size and distribution of holes, regular structure of layers, and repeatability). The fabricated scaffold in optimal conditions revealed significant antibacterial properties, good bioactivity, acceptable cell viability, and high ALP activity. 3D printed BG/PCL nanocomposite scaffolds with macro (up to 500 µm) and micro size of holes and porosity percentage up to 64% in the structure can be a promising candidate for bone tissue engineering.

کلیدواژه‌ها [English]

  • Bioactive glass
  • Bone scaffold
  • 3D print
  • Antibacterial
  • Sol-gel
  • Polycarolacton

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مواد پیشرفته در مهندسی
دوره 41، شماره 4
اسفند 1401
صفحه 1-25

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